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The application of microbeads to microfluidic systems for enhanced detection and purification of biomolecules.

Methods (San Diego, Calif.) (2016-12-15)
I F Pinto, C R F Caneira, R R G Soares, N Madaboosi, M R Aires-Barros, J P Conde, A M Azevedo, V Chu
ABSTRACT

This paper describes microbead-based microfluidic systems. Several aspects of bead assays in microfluidics make them advantageous for bioassays in simple microchannels, including enhanced surface-to-volume ratio, improved molecular recognition reaction efficiency, and the wide range of surface functionalization available with commercial microbeads. Two-level SU-8 molds are used to fabricate PDMS microchannels that can hydrodynamically trap different types of microbeads, with characteristic dimensions of tens of microns. The use of these microbead-based microfluidic systems in the biosensing of antibodies, toxins and nucleic acids, as well as in antibody purification will be presented and discussed in this paper.

MATERIALS
Product Number
Brand
Product Description

Milli-Q
Milli-Q® EQ 7000 ultrapure water purification system, Produces ultrapure (Type 1) water from pure water with a distribution flow rate up to 2 L/min., with system-mounted dispenser
Milli-Q
Milli-Q® EQ 7000 ultrapure water purification system, Produces ultrapure (Type 1) water from pure water with a distribution flow rate up to 2 L/min., with wall-mounted dispenser
Sigma-Aldrich
Propylene glycol monomethyl ether acetate, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Poly(dimethylsiloxane), viscosity 1.0 cSt (25 °C)
Sigma-Aldrich
Bovine Serum Albumin, lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture