MilliporeSigma
  • Home
  • Search Results
  • Ohr plays a central role in bacterial responses against fatty acid hydroperoxides and peroxynitrite.

Ohr plays a central role in bacterial responses against fatty acid hydroperoxides and peroxynitrite.

Proceedings of the National Academy of Sciences of the United States of America (2016-12-29)
Thiago G P Alegria, Diogo A Meireles, José R R Cussiol, Martín Hugo, Madia Trujillo, Marcos Antonio de Oliveira, Sayuri Miyamoto, Raphael F Queiroz, Napoleão Fonseca Valadares, Richard C Garratt, Rafael Radi, Paolo Di Mascio, Ohara Augusto, Luis E S Netto
ABSTRACT

Organic hydroperoxide resistance (Ohr) enzymes are unique Cys-based, lipoyl-dependent peroxidases. Here, we investigated the involvement of Ohr in bacterial responses toward distinct hydroperoxides. In silico results indicated that fatty acid (but not cholesterol) hydroperoxides docked well into the active site of Ohr from Xylella fastidiosa and were efficiently reduced by the recombinant enzyme as assessed by a lipoamide-lipoamide dehydrogenase-coupled assay. Indeed, the rate constants between Ohr and several fatty acid hydroperoxides were in the 10

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-α-Phosphatidylcholine, egg yolk, Type XVI-E, ≥99% (TLC), lyophilized powder