MilliporeSigma

Nitrated fatty acids: synthesis and measurement.

Free radical biology & medicine (2012-12-04)
Steven R Woodcock, Gustavo Bonacci, Stacy L Gelhaus, Francisco J Schopfer
ABSTRACT

Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography-mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.

MATERIALS
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