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  • Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax.

Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax.

AMB Express (2014-07-16)
Licia Lama, Annabella Tramice, Ilaria Finore, Gianluca Anzelmo, Valeria Calandrelli, Eduardo Pagnotta, Giuseppina Tommonaro, Annarita Poli, Paola Di Donato, Barbara Nicolaus, Massimo Fagnano, Mauro Mori, Adriana Impagliazzo, Antonio Trincone
ABSTRACT

Polysaccharidases from extremophiles are remarkable for specific action, resistance to different reaction conditions and other biotechnologically interesting features. In this article the action of crude extracts of thermophilic microorganisms (Thermotoga neapolitana, Geobacillus thermantarcticus and Thermoanaerobacterium thermostercoris) is studied using as substrate hemicellulose from one of the most interesting biomass crops, the giant reed (Arundo donax L.). This biomass can be cultivated without competition and a huge amount of rhizomes remains in the soil at the end of cropping cycle (10-15 years) representing a further source of useful molecules. Optimization of the procedure for preparation of the hemicellulose fraction from rhizomes of Arundo donax, is studied. Polysaccharidases from crude extracts of thermophilic microorganisms revealed to be suitable for total degradative action and/or production of small useful oligosaccharides from hemicelluloses from A. donax. Xylobiose and interesting tetra- and pentasaccharide are obtained by enzymatic action in different conditions. Convenient amount of raw material was processed per mg of crude enzymes. Raw hemicelluloses and pretreated material show antioxidant activity unlike isolated tetra- and pentasaccharide. The body of results suggest that rhizomes represent a useful raw material for the production of valuable industrial products, thus allowing to increase the economic efficiency of A. donax cultivation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-(+)-Xylose, ≥99%
Sigma-Aldrich
D-(+)-Xylose, BioXtra, ≥99%
Sigma-Aldrich
D-(+)-Xylose, ≥99%
Sigma-Aldrich
D-(+)-Xylose, BioUltra, ≥99.0% (sum of enantiomers, HPLC)
Sigma-Aldrich
Sodium propionate, ≥99.0%, BioReagent, suitable for insect cell culture
Sigma-Aldrich
Sodium propionate, ≥99.0%
Sigma-Aldrich
Sodium propionate, FCC
Sigma-Aldrich
Xylanase, powder, ≥2500 units/g, recombinant, expressed in Aspergillus oryzae
Sigma-Aldrich
Sodium propionate, meets analytical specification of NF, 99-100.5% (ex dried subst.)