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Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia.

International journal of molecular sciences (2015-06-05)
Colby A Souders, Sharon E Maynard, Jing Yan, Yang Wang, Naomi K Boatright, Jessica Sedan, David Balyozian, Peter S Cheslock, Deborah C Molrine, Tiffany A Moore Simas
ABSTRACT

Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.

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