MilliporeSigma
  • Home
  • Search Results
  • Study on interactions of aminoglycoside antibiotics with calf thymus DNA and determination of calf thymus DNA via the resonance Rayleigh scattering technique.

Study on interactions of aminoglycoside antibiotics with calf thymus DNA and determination of calf thymus DNA via the resonance Rayleigh scattering technique.

Luminescence : the journal of biological and chemical luminescence (2015-03-05)
Man Qiao, Chunyan Li, Ying Shi, Shaopu Liu, Zhongfang Liu, Xiaoli Hu
ABSTRACT

A simple and sensitive resonance Rayleigh scattering (RRS) spectra method was developed for the determination of calf thymus DNA (ctDNA). The enhanced RRS signals were based on the interactions between ctDNA and aminoglycoside antibiotics (AGs) including kanamycin (KANA), tobramycin (TOB), gentamicin (GEN) and neomycin (NEO) in a weakly acidic medium (pH 3.3-5.7). Parameters influencing the method were investigated. Under optimum conditions, increments in the scattering intensity (∆I) were directly proportional to the concentration of ctDNA over certain ranges. The detection limit ranged from 12.2 to 16.9 ng/mL. Spectroscopic methods, including RRS spectra, absorption spectra and circular dichroism (CD) spectroscopy, coupled with thermo-denaturation experiments were used to study the interactions, indicating that the interaction between AGs with ctDNA was electrostatic binding mode.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ammonium chloride, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Aluminum chloride solution, 1.0 M in nitrobenzene
Sigma-Aldrich
Tin(II) chloride, reagent grade, 98%
Sigma-Aldrich
Aluminum chloride, ReagentPlus®, 99%
Sigma-Aldrich
Aluminum chloride, anhydrous, sublimed, ≥98%
Sigma-Aldrich
Aluminum chloride, reagent grade, 98%
Sigma-Aldrich
Ammonium chloride, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.5-100.5% (calc. to the dried substance)
Sigma-Aldrich
Aluminum chloride, anhydrous, powder, 99.99% trace metals basis
Sigma-Aldrich
Tin(II) chloride, anhydrous, powder, ≥99.99% trace metals basis
Sigma-Aldrich
Tin(II) chloride, ≥99.99% trace metals basis
Sigma-Aldrich
D-(−)-Fructose, BioUltra, ≥99.0% (HPLC)
Sigma-Aldrich
Ammonium chloride, 99.99% trace metals basis
SAFC
L-Histidine
Sigma-Aldrich
Aluminum chloride, anhydrous, powder, 99.999% trace metals basis
Sigma-Aldrich
L-Histidine, suitable for cell culture, meets EP, USP testing specifications, from non-animal source
Sigma-Aldrich
L-Histidine, ReagentPlus®, ≥99% (TLC)
Sigma-Aldrich
Ammonium chloride, for molecular biology, suitable for cell culture, ≥99.5%
Sigma-Aldrich
D-(−)-Fructose, BioXtra
Sigma-Aldrich
D-(−)-Fructose, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
D-(−)-Fructose, ≥99%
Sigma-Aldrich
D-(−)-Fructose, meets USP testing specifications
Sigma-Aldrich
Ammonium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
L-Histidine, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
Aluminum chloride, 99.99% trace metals basis
Sigma-Aldrich
Ammonium chloride, 99.998% trace metals basis
Sigma-Aldrich
Kanamycin sulfate from Streptomyces kanamyceticus, powder, BioReagent, suitable for cell culture, suitable for plant cell culture
Sigma-Aldrich
L-Tryptophan, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 99.0-101.0%
Sigma-Aldrich
L-Tryptophan, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
Kanamycin sulfate Streptomyces kanamyceticus, powder, γ-irradiated
Sigma-Aldrich
Kanamycin sulfate from Streptomyces kanamyceticus, meets USP testing specifications, powder