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Bioconversion of xylose, hexoses and biomass to ethanol by a new isolate of the white rot basidiomycete Trametes versicolor.

SpringerPlus (2014-03-14)
Kenji Okamoto, Atsushi Uchii, Ryuichi Kanawaku, Hideshi Yanase
ABSTRACT

Second-generation bioethanol production requires the development of economically feasible and sustainable processes that use renewable lignocellulosic biomass as a starting material. However, the microbial fermentation of xylose, which is the principal pentose sugar in hemicellulose, is a limiting factor in developing such processes. Here, a strain of the white rot basidiomycete Trametes versicolor that was capable of efficiently fermenting xylose was newly isolated and characterized. This strain, designated KT9427, was capable of assimilating and converting xylose to ethanol under anaerobic conditions with a yield of 0.44 g ethanol per 1 g of sugar consumed. In culture medium containing low yeast extract concentrations, xylose consumption and ethanol productivity were enhanced. Adjusting the initial pH between 3.0 and 5.0 did not markedly influence xylose fermentation. T. versicolor KT9427 also produced ethanol from glucose, mannose, fructose, cellobiose and maltose at yields ranging from 0.45 to 0.49 g ethanol per 1 g of sugar consumed. In addition, strain KT9427 exhibited favourable conversion of non-pretreated starch, cellulose, xylan, wheat bran and rice straw into ethanol compared to common recombinant yeast strains. Taken together, the present findings suggest that T. versicolor KT9427 is a promising candidate for environmentally friendly ethanol production directly from lignocellulosic biomass.

MATERIALS
Product Number
Brand
Product Description

Supelco
Ethanol standards 10% (v/v), 10 % (v/v) in H2O, analytical standard
Sigma-Aldrich
D-(+)-Mannose, wood, ≥99%
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D-(+)-Mannose, synthetic, ≥99%
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D-(+)-Glucose, ≥99.5% (GC)
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D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)
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D-(+)-Glucose, ACS reagent
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D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
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D-(+)-Glucose, BioXtra, ≥99.5% (GC)
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D-(+)-Glucose, Hybri-Max, powder, BioReagent, suitable for hybridoma
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D-Glucose-12C6, 16O6, 99.9 atom % 16O, 99.9 atom % 12C
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Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
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Magnesium sulfate solution, for molecular biology, 1.00 M±0.04 M
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D-(+)-Mannose, powder, BioReagent, suitable for cell culture
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D-(−)-Fructose, BioXtra
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D-(−)-Fructose, ≥99%
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D-(−)-Fructose, meets USP testing specifications
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D-(−)-Fructose, BioReagent, suitable for cell culture, suitable for insect cell culture
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Magnesium sulfate solution, BioUltra, for molecular biology
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D-(+)-Mannose, BioUltra, ≥99.5% (sum of enantiomers, HPLC)
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D-(−)-Fructose, BioUltra, ≥99.0% (HPLC)
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D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
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Dextrose, meets EP, BP, JP, USP testing specifications, anhydrous
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Potassium phosphate monobasic, ReagentPlus®
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Potassium phosphate monobasic, for molecular biology, ≥98.0%
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Potassium phosphate monobasic, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
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D-(+)-Xylose, ≥99%
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D-(+)-Xylose, BioXtra, ≥99%
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Magnesium sulfate, BioReagent, suitable for cell culture, suitable for insect cell culture
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Ethanol, anhydrous, denatured
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Ethyl alcohol, Pure, 190 proof, meets USP testing specifications