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A strategy for dissecting the architectures of native macromolecular assemblies.

Nature methods (2015-10-06)
Yi Shi, Riccardo Pellarin, Peter C Fridy, Javier Fernandez-Martinez, Mary K Thompson, Yinyin Li, Qing Jun Wang, Andrej Sali, Michael P Rout, Brian T Chait
ABSTRACT

It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.

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