MilliporeSigma
  • Home
  • Search Results
  • Antioxidant and anti-ageing activities of mycelia zinc polysaccharide from Pholiota nameko SW-03.

Antioxidant and anti-ageing activities of mycelia zinc polysaccharide from Pholiota nameko SW-03.

Journal of the science of food and agriculture (2014-12-17)
Lan Zheng, Min Liu, Guo-Yin Zhai, Zhao Ma, Li-Qin Wang, Le Jia
ABSTRACT

Edible fungi polysaccharides usually exhibit antioxidant activity, and zinc has been shown to have antioxidant properties. In the present work, Pholiota nameko SW-03 was used as a vector of zinc biotransformation in order to obtain mycelia zinc polysaccharide (MZPS), and the structural characterization and anti-ageing activity of MZPS were investigated. Pholiota nameko SW-03 could accumulate zinc in the form of zinc-enriched polysaccharide, and the zinc content in MZPS was 16.39 ± 0.72 mg g(-1) . Three fractions (MZPS-1, MZPS-2 and MZPS-3) were successfully isolated. The main fraction (MZPS-2) with the highest antioxidant activity in vitro was composed of glucose, mannose, glucuronic acid, galactose, galacturonic acid and arabinose in a molar ratio of 172.59:5.29:4.61:4.20:1.01:1.00, with a weight-average molecular weight of 13.63 kDa. The anti-ageing capability was measured in d-galactose-induced ageing mice, and the results showed that MZPS could improve antioxidant status (superoxide dismutase, total antioxidant capability, malondialdehyde and lipid peroxide), indicating that MZPS had strong anti-ageing capability in vivo. This study suggested that organification of zinc through edible fungi liquid fermentation provided a novel method to produce MZPS, which might be used as a natural antioxidant to slow the progression of ageing.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-(+)-Mannose, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
D-Glucuronic acid, ≥98% (GC)
Sigma-Aldrich
D-(+)-Galactose, BioXtra, pH 5.0-7.0 (20 °C, 1 M in H2O), ≥99%
Sigma-Aldrich
D-(+)-Galactose, ≥98%
Sigma-Aldrich
D-(+)-Galactose, ≥99%
Sigma-Aldrich
D-(−)-Arabinose, ≥98%
Sigma-Aldrich
D-(+)-Mannose, BioUltra, ≥99.5% (sum of enantiomers, HPLC)
Sigma-Aldrich
Potassium bromide, BioUltra, ≥99.5% (AT)
Sigma-Aldrich
Potassium bromide, anhydrous, powder, 99.95% trace metals basis
Sigma-Aldrich
Potassium bromide, anhydrous, powder, 99.999% trace metals basis
Sigma-Aldrich
D-(+)-Galactose, powder, anhydrous, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
Potassium bromide, BioXtra, ≥99.0%
Sigma-Aldrich
D-(+)-Mannose, wood, ≥99%
SAFC
Galactose, plant-derived
Sigma-Aldrich
Potassium bromide, ACS reagent, ≥99.0%
Sigma-Aldrich
Potassium bromide, FT-IR grade, ≥99% trace metals basis
Sigma-Aldrich
Potassium bromide, anhydrous, free-flowing, Redi-Dri, ReagentPlus®, ≥99%
Sigma-Aldrich
D-(+)-Mannose, synthetic, ≥99%
Sigma-Aldrich
Potassium bromide, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Potassium bromide, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99%
Sigma-Aldrich
Sucrose, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, ACS reagent
Sigma-Aldrich
Sucrose, meets USP testing specifications
Sigma-Aldrich
Sucrose, Grade I, suitable for plant cell culture
Sigma-Aldrich
Sucrose, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
Sigma-Aldrich
Sucrose, ≥99.5%
Sigma-Aldrich
Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF
Sigma-Aldrich
Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)