MilliporeSigma
  • Home
  • Search Results
  • Galleria mellonella Larvae as an Infection Model for Penicillium marneffei.

Galleria mellonella Larvae as an Infection Model for Penicillium marneffei.

Mycopathologia (2015-05-25)
Xiaowen Huang, Dedong Li, Liyan Xi, Eleftherios Mylonakis
ABSTRACT

Penicillium marneffei, the only known dimorphic and pathogenic species in the genus of Penicillium, is responsible for severe to deadly infection in immunocompromised patients. In this study, P. marneffei was able to infect the greater wax moth Galleria mellonella. The increasing inoculum doses of P. marneffei cells resulted in greater larval mortality, and the larval mortality rate also depended on the incubation temperature after P. marneffei infection and different P. marneffei strains. Moreover, the phagocytosis of hemocytes to P. marneffei was investigated, and it showed that the phagocytosis was increasing during the infection. These results demonstrated that G. mellonella can be effectively used to facilitate the in vivo study of P. marneffei infection and hemocytes are the key component of the larvae's immune defenses.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
Sigma-Aldrich
Trypan Blue, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
Sigma-Aldrich
Trypan Blue, Dye content 60 %
Sigma-Aldrich
Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)
Sigma-Aldrich
Trypan Blue solution, 0.4%, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Fluorescein 5(6)-isothiocyanate, BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)