Tri-layered chitosan scaffold as a potential skin substitute.

Journal of biomaterials science. Polymer edition (2015-07-15)
Hsin-Yi Lin, Shin-Hung Chen, Shih-Hsin Chang, Sheng-Tung Huang

A tri-layered chitosan-based scaffold was successfully made to replicate the striation of a full-thickness skin more accurately than a single- or bi-layered scaffold, which needed weeks of co-culturing of fibroblasts and keratinocytes to achieve similar striation. Chitosan solution was freeze-dried and made into porous disks. Chitosan or chitosan-pectin in acetic acid solution was electrospun onto the chitosan disk to form a nanofibrous layer and a thin film. Examinations based on scanning electron spectroscopy showed that the scaffold was composed of a porous layer (2 mm) to simulate the dermis, a thin film (25-45 μm) to mimic the basement membrane, and a layer of nanofibers (100-200 μm) to serve as the protective epidermis. The tensile strength and modulus of the composite scaffold were significantly higher than those of the chitosan disk (p < 0.01). The composite was able to quickly absorb water and stayed intact throughout the course of the 14-day cell culture tests. The fibroblast cells seeded on both sides of the scaffolds were able to proliferate and stayed separated by the thin film.

Product Number
Product Description

5α-Androstan-17β-ol-3-one, ≥97.5%
Hexamethyldisilazane, ReagentPlus®, 99.9%
Hexamethyldisilazane, reagent grade, ≥99%
Acetic acid-12C2, 99.9 atom % 12C
Acetic acid, ≥99.5%, FCC, FG
Acetic acid, natural, ≥99.5%, FG
Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)