MilliporeSigma
  • Home
  • Search Results
  • Revealing the characteristics of a novel bioflocculant and its flocculation performance in Microcystis aeruginosa removal.

Revealing the characteristics of a novel bioflocculant and its flocculation performance in Microcystis aeruginosa removal.

Scientific reports (2015-12-03)
Pengfei Sun, Cai Hui, Naling Bai, Shengmao Yang, Li Wan, Qichun Zhang, YuHua Zhao
ABSTRACT

In the present work, a novel bioflocculant, EPS-1, was prepared and used to flocculate the kaolin suspension and Microcystis aeruginosa. We focused on the characteristics and flocculation performance of EPS-1, especially with regard to its protein components. An important attribute of EPS-1 was its protein content, with 18 protein types identified that occupied a total content of 31.70% in the EPS-1. Moreover, the flocculating activity of these protein components was estimated to be no less than 33.93%. Additionally, polysaccharides that occupied 57.12% of the total EPS-1 content consisted of four monosaccharides: maltose, D-xylose, mannose, and D-fructose. In addition, carbonyl, amino, and hydroxyl groups were identified as the main functional groups. Three main elements, namely C1s, N1s, and O1s, were present in EPS-1 with relative atomic percentages of 62.63%, 24.91%, and 10.5%, respectively. Zeta potential analysis indicated that charge neutralization contributed to kaolin flocculation, but was not involved in M. aeruginosa flocculation. The flocculation conditions of EPS-1 were optimized, and the maximum flocculating efficiencies were 93.34% within 2 min for kaolin suspension and 87.98% within 10 min for M. aeruginosa. These results suggest that EPS-1 could be an alternative to chemical flocculants for treating wastewaters and cyanobacterium-polluted freshwater.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-(+)-Xylose, ≥99%
Sigma-Aldrich
D-(+)-Xylose, BioXtra, ≥99%
Sigma-Aldrich
Maltose solution, BioReagent, for molecular biology, ~20% in H2O
Sigma-Aldrich
D-(+)-Xylose, BioUltra, ≥99.0% (sum of enantiomers, HPLC)
Sigma-Aldrich
L-Rhamnose, natural sourced, 99%, FG
Sigma-Aldrich
D-(+)-Xylose, ≥99%
Sigma-Aldrich
Dextrose, meets EP, BP, JP, USP testing specifications, anhydrous
Sigma-Aldrich
D-Glucose-12C6, 16O6, 99.9 atom % 16O, 99.9 atom % 12C
Supelco
D-(+)-Glucose, analytical standard
Sigma-Aldrich
D-(+)-Mannose, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
D-(+)-Mannose, BioUltra, ≥99.5% (sum of enantiomers, HPLC)
Sigma-Aldrich
D-(−)-Fructose, ≥99%
Sigma-Aldrich
D-(−)-Fructose, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
D-(−)-Fructose, meets USP testing specifications
Sigma-Aldrich
D-(−)-Fructose, BioXtra
Sigma-Aldrich
D-(+)-Glucose, ≥99.5% (GC)
Sigma-Aldrich
D-(+)-Galactose, ≥99%
Sigma-Aldrich
D-(+)-Glucose, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
D-(+)-Galactose, BioXtra, pH 5.0-7.0 (20 °C, 1 M in H2O), ≥99%
Sigma-Aldrich
D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)
Sigma-Aldrich
D-(+)-Glucose, ACS reagent
Sigma-Aldrich
D-(−)-Fructose, BioUltra, ≥99.0% (HPLC)
Sigma-Aldrich
D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
Sigma-Aldrich
D-(+)-Galactose, ≥98%
Sigma-Aldrich
D-(+)-Galactose, powder, anhydrous, BioReagent, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Sigma-Aldrich
D-(+)-Glucose, Hybri-Max, powder, BioReagent, suitable for hybridoma
SAFC
Galactose, plant-derived
Sigma-Aldrich
D-(+)-Mannose, wood, ≥99%
Sigma-Aldrich
D-(+)-Mannose, synthetic, ≥99%