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SPIM-fluid: open source light-sheet based platform for high-throughput imaging.

Biomedical optics express (2015-11-26)
Emilio J Gualda, Hugo Pereira, Tiago Vale, Marta Falcão Estrada, Catarina Brito, Nuno Moreno
ABSTRACT

Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an open source unified implementation based on Arduino and Micromanager, which is capable of operating Light Sheet Microscopes for automatized 3D high-throughput imaging on three-dimensional cell cultures and model organisms like zebrafish, oriented to massive drug screening.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Mercury, electronic grade, 99.9999% trace metals basis
Sigma-Aldrich
Mercury, ≥99.99% trace metals basis
Sigma-Aldrich
Mercury, ACS reagent, 99.9995% trace metals basis