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Formation of redox-active self-assembled polyelectrolyte-surfactant complexes integrating glucose oxidase on electrodes: Influence of the self-assembly solvent on the signal generation.

Bioelectrochemistry (Amsterdam, Netherlands) (2015-06-22)
M Lorena Cortez, Marcelo Ceolín, Omar Azzaroni, Fernando Battaglini
ABSTRACT

In this work the effects of the self-assembly solvent on the structure and electrochemical behavior of redox-active polyelectrolyte–surfactant complexes cast on electrode supports from aqueous and DMF solutions are presented. The complex studied is formed by complexation of osmium complex-modified polyallylamine (OsPA) with dodecyl sulfate (DS) surfactants. The structure of the films was characterized by GISAXS, showing that films present a lamellar mesostructure. However, when they are exposed to humid environments, films cast from aqueous solutions (OsPA–DSaq) undergo a structural transition that ultimately leads to the disappearance of the mesostructural order. On the other hand, OsPA–DS films cast from DMF solutions (OsPA–DSorg) revealed no significant changes upon exposure to humid environments. Both types of films were exposed to glucose oxidase (GOx), showing similar adsorption characteristics. Notwithstanding these similarities in GOx and content, OsPA–DSaq films revealed a more sensitive bioelectrocatalytical response to glucose as compared to OsPA–DSorg films.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
N,N-Dimethylformamide, suitable for HPLC, ≥99.9%
Supelco
Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)
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Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis
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Trizma® base, BioXtra, pH 10.5-12.0 (1 M in H2O), ≥99.9% (titration)
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Trizma® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline
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Trizma® base, ≥99.9% (titration), crystalline
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Trizma® base, BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
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N,N-Dimethylformamide, for molecular biology, ≥99%
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Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
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D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)
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D-(+)-Glucose, ≥99.5% (GC)
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D-(+)-Glucose, ACS reagent
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D-(+)-Glucose, BioXtra, ≥99.5% (GC)
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D-(+)-Glucose, Hybri-Max, powder, BioReagent, suitable for hybridoma
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D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
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Sodium dodecyl sulfate, ReagentPlus®, ≥98.5% (GC)
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Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)
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D-Glucose-12C6, 16O6, 99.9 atom % 16O, 99.9 atom % 12C
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Sodium dodecyl sulfate, ACS reagent, ≥99.0%
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N,N-Dimethylformamide, anhydrous, 99.8%
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Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%
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N,N-Dimethylformamide, ACS reagent, ≥99.8%
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Sodium dodecyl sulfate, ≥90%
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Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate
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D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
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Trizma® base, puriss. p.a., ≥99.7% (T)
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Trizma® base, ≥99.0% (T)
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Sodium dodecyl sulfate, ≥98.0% (GC)
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Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)
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Trizma® base, BioUltra, for molecular biology, ≥99.8% (T)