MilliporeSigma
  • Home
  • Search Results
  • Reduction of molecular leaching from a gel matrix for the precisely controlled release of encapsulated molecules by light stimulus.

Reduction of molecular leaching from a gel matrix for the precisely controlled release of encapsulated molecules by light stimulus.

Chemical & pharmaceutical bulletin (2014-07-06)
Takaki Amamoto, Tomofumi Santa, Masaru Kato
ABSTRACT

We have developed a general method for controlling molecular functions using a photodegradable hydrogel; gels containing molecules made from such materials are capable of release and activation by light stimulus. As the elimination of molecular leaching from the gel before irradiation was a barrier to the precise control of molecular function, we optimized the monomer used in gel preparation during this study. The addition of N,N'-methylenebis(acrylamide) (MBAA) inhibited molecular leaching from the gel; the MBAA concentration is a critical factor in controlling the leaching of encapsulated molecules. We succeeded in preparing a gel that halved the leaching of small encapsulated molecules, while the leaching of large molecules, such as albumin (66 kDa) and ferritin (450 kDa), was at negligible levels, or disappeared. The on/off ratios (released amount/leached amount) of albumin and ferritin were 8 and 17, respectively.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ammonium persulfate, ACS reagent, ≥98.0%
Sigma-Aldrich
Ammonium persulfate, reagent grade, 98%
Sigma-Aldrich
Ammonium persulfate, free-flowing, Redi-Dri, reagent grade, 98%
Supelco
Dichloromethane solution, certified reference material, 200 μg/mL in methanol
Supelco
Dichloromethane, analytical standard, stabilized
Sigma-Aldrich
N,N,N′,N′-Tetramethylethylenediamine, ReagentPlus®, 99%
Sigma-Aldrich
N,N,N′,N′-Tetramethylethylenediamine, ≥99.5%, purified by redistillation
Supelco
Dichloromethane, analytical standard
Sigma-Aldrich
4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride, ≥96.0% (calc. on dry substance, AT)
Supelco
Dichloromethane, Selectophore, ≥99.5%
Sigma-Aldrich
Ammonium persulfate, BioUltra, for molecular biology, ≥98.0% (RT)
Sigma-Aldrich
N,N,N′,N′-Tetramethylethylenediamine, BioReagent, for molecular biology, ≥99% (GC)
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
Sigma-Aldrich
Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)
Sigma-Aldrich
N,N,N′,N′-Tetramethylethylenediamine, BioReagent, suitable for electrophoresis, ~99%
Sigma-Aldrich
Acrylamide solution, 40%, suitable for electrophoresis, sterile-filtered
Sigma-Aldrich
Ammonium persulfate, for molecular biology, suitable for electrophoresis, ≥98%
Trometamol, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Ammonium persulfate, BioXtra, ≥98.0%
Sigma-Aldrich
N,N′-Methylenebisacrylamide solution, suitable for electrophoresis, 2% in H2O
Sigma-Aldrich
Tromethamine, meets USP testing specifications
Sigma-Aldrich
Sigma 7-9®, ≥99% (titration), crystalline
Sigma-Aldrich
Dichloromethane, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
Dichloromethane, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 50-150 ppm amylene as stabilizer
Sigma-Aldrich
Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer
Supelco
Acrylamide solution, 40% in H2O, for molecular biology
Sigma-Aldrich
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
Supelco
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 5 mm × 8 in.
Sigma-Aldrich
Dichloromethane, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%
Sigma-Aldrich
Dichloromethane, biotech. grade, 99.9%, contains 40-150 ppm amylene as stabilizer