MilliporeSigma
  • Home
  • Search Results
  • The effect of nanoliposomal formulations on Staphylococcus epidermidis biofilm.

The effect of nanoliposomal formulations on Staphylococcus epidermidis biofilm.

Drug development and industrial pharmacy (2014-01-11)
Niloofar Moghadas-Sharif, Bibi Sedigheh Fazly Bazzaz, Bahman Khameneh, Bizhan Malaekeh-Nikouei
ABSTRACT

The aim of the present study was to assess the in vitro antimicrobial activities of nanoliposomal formulations loaded with vancomycin or/and rifampin against the biofilm formed by Staphylococcus epidermidis at 37 °C under aerobic condition. Liposomal formulations were prepared by dehydration-rehydration (DRV) method and characterized for size, zeta potential and encapsulation efficacy. The ability of different formulations on eradication of bacterial biofilm was assessed through optical density ratio (ODr) and the results implicate higher survival rates of S. epidermidis on biofilm. Positive control was defined as an ODr = 1.0. The zeta potential of anionic, cationic and PEGylated liposomes was -35 ± 2, 35 ± 1 and 27 ± 2 mV whereas the mean sizes of these liposomal formulations were 145 ± 4, 134 ± 1 and 142 ± 6 nm, respectively. Encapsulation efficacy of rifampin and vancomycin was more than 60% and about 25%, respectively. Cationic liposomal rifampin lowered the ODr to 0.61 and was the most effective formulations against S. epidermidis biofilm (p<0.001). The antibiofilm activity of liposomal formulations was concentration- and time-dependent manner. The results of this study showed that rifampin-loaded liposomes were effective against bacterial biofilm.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2,3,5-Triphenyltetrazolium chloride, ≥98.0% (HPLC)
Supelco
Cholesterol solution, certified reference material, 10 mg/mL in chloroform
Sigma-Aldrich
SyntheChol® NS0 Supplement, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer
Sigma-Aldrich
Dihexadecyl phosphate
Sigma-Aldrich
Cholesterol, Sigma Grade, ≥99%
Sigma-Aldrich
Cholesterol, from sheep wool, ≥92.5% (GC), powder
Sigma-Aldrich
Cholesterol, powder, BioReagent, suitable for cell culture, ≥99%
Sigma-Aldrich
Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer
Supelco
Chloroform, suitable for HPLC, ≥99.8%
Sigma-Aldrich
Octadecylamine, ≥99% (GC)
Sigma-Aldrich
Chloroform, ACS reagent, ≥99.8%, contains amylenes as stabilizer
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, 99.93%
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Supelco
Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%
Supelco
Chloroform, analytical standard
Supelco
Methanol, analytical standard
Sigma-Aldrich
Chloroform, ACS reagent, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
Sigma-Aldrich
Octadecylamine, technical grade, 90%
Sigma-Aldrich
Octadecylamine, ≥99.0% (GC)
Sigma-Aldrich
Methanol, NMR reference standard
Sigma-Aldrich
Titanium tetrachloride, packaged for use in deposition systems
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
Supelco
Methanol, Pharmaceutical Secondary Standard; Certified Reference Material
SAFC
Cholesterol, from sheep wool, Controlled origin, meets USP/NF testing specifications
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Titanium(IV) chloride solution, 1.0 M in toluene
Sigma-Aldrich
Chloroform, puriss. p.a., reag. ISO, reag. Ph. Eur., 99.0-99.4% (GC)
Sigma-Aldrich
Methanol, puriss., meets analytical specification of Ph Eur, ≥99.7% (GC)