MilliporeSigma

A manual and an automatic TERS based virus discrimination.

Nanoscale (2015-02-17)
Konstanze Olschewski, Evelyn Kämmer, Stephan Stöckel, Thomas Bocklitz, Tanja Deckert-Gaudig, Roland Zell, Dana Cialla-May, Karina Weber, Volker Deckert, Jürgen Popp
ABSTRACT

Rapid techniques for virus identification are more relevant today than ever. Conventional virus detection and identification strategies generally rest upon various microbiological methods and genomic approaches, which are not suited for the analysis of single virus particles. In contrast, the highly sensitive spectroscopic technique tip-enhanced Raman spectroscopy (TERS) allows the characterisation of biological nano-structures like virions on a single-particle level. In this study, the feasibility of TERS in combination with chemometrics to discriminate two pathogenic viruses, Varicella-zoster virus (VZV) and Porcine teschovirus (PTV), was investigated. In a first step, chemometric methods transformed the spectral data in such a way that a rapid visual discrimination of the two examined viruses was enabled. In a further step, these methods were utilised to perform an automatic quality rating of the measured spectra. Spectra that passed this test were eventually used to calculate a classification model, through which a successful discrimination of the two viral species based on TERS spectra of single virus particles was also realised with a classification accuracy of 91%.

MATERIALS
Product Number
Brand
Product Description

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