Oxidative stress in songbirds exposed to dietary methylmercury.

Ecotoxicology (London, England) (2014-12-19)
Katie A Henry, Daniel A Cristol, Claire W Varian-Ramos, Eric L Bradley

Long-term, sublethal methylmercury exposure can cause reproductive depression, immune suppression, endocrine disruption and other problems in birds. We used two biomarkers to detect oxidative stress in livers of zebra finches (Taeniopygia guttata) developmentally exposed to sublethal levels of dietary methylmercury (0.0, 0.3, 0.6, 1.2, or 2.4 μg/g wet weight in diet). Our findings indicate that young adult finches exposed to environmentally relevant concentrations of mercury in ovo and through their diets, exhibited oxidative stress in their livers. We measured the ratio of the antioxidant glutathione in its reduced form (GSH) versus its oxidized form (GSSG) and the activity of the superoxide dismutase (SOD) enzyme suite. Blood total mercury served as a proxy for liver mercury concentration, and was on average 8.4 times the dietary dose (e.g., birds consuming 0.6 μg/g had blood mercury levels of ~5 μg/g on a wet weight basis). Consistent with what is known from large, aquatic bird species, there was a significant, negative relationship between GSH/GSSG ratios and tissue mercury concentrations, which is indicative of oxidative stress. This relationship was driven by a significant increase in the oxidized glutathione in the livers of birds with higher blood mercury levels. SOD activity was also found to have a significant, negative relationship with blood mercury.

Product Number
Product Description

5-(N,N-Dimethyl)amiloride hydrochloride
L-Glutathione reduced, BioXtra, ≥98.0%
L-Glutathione reduced, suitable for cell culture, BioReagent, ≥98.0%, powder
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Mercury, electronic grade, 99.9999% trace metals basis
L-Glutathione oxidized disodium salt, ≥98%, powder
L-Glutathione oxidized disodium salt, suitable for cell culture, BioReagent
Mercury, ≥99.99% trace metals basis
Mercury, ACS reagent, 99.9995% trace metals basis
Glutathione, European Pharmacopoeia (EP) Reference Standard
Glutathione, Pharmaceutical Secondary Standard; Certified Reference Material