• Home
  • Search Results
  • Anti-inflammatory effects of vitamin E on adjuvant-induced arthritis in rats.

Anti-inflammatory effects of vitamin E on adjuvant-induced arthritis in rats.

Inflammation (2014-08-15)
Mateus Fortes Rossato, Carin Hoffmeister, Raquel Tonello, Ana Paula de Oliveira Ferreira, Juliano Ferreira
ABSTRACT

Vitamin E (vit-E) is a lipophilic antioxidant, and its anti-inflammatory activity is still not full characterized. Thus, our goal was to investigate the anti-inflammatory effect of repeated vit-E treatment in the arthritis induced by the intraplantar injection of complete Freund's adjuvant (CFA). We observed an increase in arthritis scores, interleukin-1β and H2O2 levels, neutrophil and macrophage infiltration, thermal hyperalgesia, mechanical allodynia, and loss of function induced by intraplantar CFA injection. These effects were unaltered after 1 day, partially reversed after 3 days, and inhibited after 9 days after vit-E treatment. Furthermore, the concentration of vit-E was reduced and that of tumor necrosis factor-alpha was increased in the CFA-injected paw. Both effects were reversed from 1 to 9 days after vit-E treatment. However, vit-E treatment did not alter CFA-induced edema at any time. Thus, vit-E treatment produced an anti-inflammatory effect of slow onset in CFA, which demonstrates a disease-modifying drug profile.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hexane, suitable for HPLC, ≥97.0% (GC)
Sigma-Aldrich
Hexane, anhydrous, 95%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥98%
Sigma-Aldrich
Hexane, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99% (GC)
Sigma-Aldrich
2,6-Di-tert-butyl-4-methylphenol, ≥99.0% (GC), powder
Sigma-Aldrich
Butylated hydroxytoluene, ≥99%, FCC, FG
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, for molecular biology, ≥99%
Sigma-Aldrich
Hexane, suitable for HPLC, ≥95%
Supelco
Butylated Hydroxytoluene, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
3,3′,5,5′-Tetramethylbenzidine, ≥99%
Sigma-Aldrich
Hexane, HPLC Plus, for HPLC, GC, and residue analysis, ≥95%
Sigma-Aldrich
Phenol Red, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Hexane, ReagentPlus®, ≥99%
Sigma-Aldrich
Hexane, Laboratory Reagent, ≥95%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, BioXtra, ≥99%
Sigma-Aldrich
Phenol Red, ACS reagent
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, BioUltra, for molecular biology, ≥99.0% (AT)
Sigma-Aldrich
3,3′,5,5′-Tetramethylbenzidine, ≥98% (TLC)
Sigma-Aldrich
2,6-Di-tert-butyl-4-methylphenol, purum, ≥99.0% (GC)
Supelco
Hexane, analytical standard
Sigma-Aldrich
3,3′,5,5′-Tetramethylbenzidine, ≥98.0% (NT)
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥96.0% (AT)
USP
Butylated hydroxytoluene, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet
Supelco
Hexadecyltrimethylammonium bromide, suitable for ion pair chromatography, LiChropur
USP
Cetrimonium bromide, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
2,6-Di-tert-butyl-4-methylphenol, tested according to Ph. Eur.
Sigma-Aldrich
Hexane, HPLC Plus, for HPLC, GC, and residue analysis, ≥95%
Supelco
Hexadecyltrimethylammonium bromide, analytical standard
Supelco
3,3′,5,5′-Tetramethylbenzidine, standard for GC