• Home
  • Search Results

  • Analysis of whole blood samples with low gas flow inductively coupled plasma-optical emission spectrometry.

Analysis of whole blood samples with low gas flow inductively coupled plasma-optical emission spectrometry.

Analytical and bioanalytical chemistry (2014-09-23)
Sascha Nowak, Jens Künnemeyer, Lydia Terborg, Stefan Trümpler, Andreas Günsel, Gerhard A Wiesmüller, Uwe Karst, Wolfgang Buscher
PMID25240935
ABSTRACT

Low gas flow ICP-OES with a total argon consumption below 0.7 L/min is introduced for the analysis of trace elements in blood samples to investigate the influence of samples containing an organic solvent in a demanding matrix on the performance of this plasma for the first time. Therefore, gadolinium was determined in human plasma samples and mercury in red blood cells, human plasma, and precipitated plasma protein fraction. Limits of detection (LOD) were determined to be in the low microgram per liter range for the analytes and the accuracy of the method was assessed by comparison with a conventional Fassel-type torch-based ICP-OES. It was proven that the low gas flow ICP-OES leads to comparable results with the instrument based on the Fassel-type torch.

MATERIALS
Product Number
Brand
Product Description
34851
Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
271004
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
34998
Sigma-Aldrich
Acetonitrile, HPLC Plus, ≥99.9%
438073
Sigma-Aldrich
Nitric acid, ACS reagent, 70%
440191
Sigma-Aldrich
Hexamethyldisilazane, reagent grade, ≥99%
225711
Sigma-Aldrich
Nitric acid, 70%, purified by redistillation, ≥99.999% trace metals basis
439134
Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
574732
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
360457
Sigma-Aldrich
Acetonitrile, ACS reagent, ≥99.5%
30709
Sigma-Aldrich
Nitric acid, puriss. p.a., reag. ISO, reag. Ph. Eur., for determinations with dithizone, ≥65%
84378
Sigma-Aldrich
Nitric acid, puriss. p.a., 65.0-67.0%
34881
Sigma-Aldrich
Acetonitrile, suitable for HPLC-GC, ≥99.8% (GC)
695041
Sigma-Aldrich
Nitric acid, ACS reagent, ≥90.0%
52619
Supelco
Hexamethyldisilazane, for GC derivatization, LiChropur™, ≥99.0% (GC)
379212
Sigma-Aldrich
Hexamethyldisilazane, ReagentPlus®, 99.9%
16355
Supelco
Nitric acid concentrate, 0.1 M HNO3 in water (0.1N), eluent concentrate for IC
576956
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) formic acid, suitable for HPLC
309079
Sigma-Aldrich
Nitric acid, red, fuming, HNO3 >90 %
675415
Sigma-Aldrich
Acetonitrile, HPLC Plus, ≥99.9%, poly-coated bottles
PHR1551
Supelco
Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
84380
Sigma-Aldrich
Nitric acid, puriss. p.a., ≥65% (T)
86944
Sigma-Aldrich
Hexamethyldisilazane, produced by Wacker Chemie AG, Burghausen, Germany, ≥97.0% (GC)
494445
Sigma-Aldrich
Acetonitrile, biotech. grade, ≥99.93%
45983
Supelco
Acetonitrile, analytical standard
110086
Sigma-Aldrich
Acetonitrile, ReagentPlus®, 99%
733466
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
34442
Sigma-Aldrich
Acetonitrile, suitable for DNA synthesis, ≥99.9% (GC)
PHR1320
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
679186
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (w/v) ammonium formate, 0.1 % (v/v) formic acid, 5 % (v/v) water, suitable for HPLC
33019
Sigma-Aldrich
Acetonitrile, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.5% (GC)