Serum free fatty acids level in senile cataract.

Journal of the American College of Nutrition (2014-08-01)
Dong Chang, Shengzhong Rong, Yannan Zhang, Qian Sha, Meihua Liang, Xuefei Zhang, Miaojing Li, Hongzhi Pan
ABSTRACT

To evaluate and compare the levels of free fatty acids between senile cataract patients and normal controls. Fifty consecutive patients with newly diagnosed senile cataract and 50 age- and gender-matched controls were evaluated. Subjects/patients were randomized according to selection criteria. The levels of free fatty acids (FFAs) in serum were measured by gas chromatography-mass spectrometry (GC-MS). Sixteen fatty acids from 14:0 to 24:1 were identified. The values were compared between cataract and control groups by parametric independent sample test and Mann-Whitney U tests. A significant decrease was observed in arachidonic acid (C20:4n-6, ARA), cis-4,7,10,13,16,19-docosahexaenoic acid (C22:6n-3, DHA), tetracosanoic acid (C24: 0), cis-7,10,13,16,19-docosapentaenoic acid (C22:5n-6, DPA), total n-3 long-chain polyunsaturated fatty acids (LC-PUFAs), total n-6 LC-PUFAs, total fatty acids, unsaturated fatty acids (USFAs), polyunsaturated fatty acids (PUFAs), and nonessential fatty acid levels in patients with senile cataract in comparison with healthy persons (p < 0.05). The levels of FFA including DPA, tetracosanoic acid, ARA, and DHA were significantly lower in the senile cataract group compared to that in the normal controls. FFA may be helpful in preventing senile cataract.

MATERIALS
Product Number
Brand
Product Description

USP
Palmitic acid, United States Pharmacopeia (USP) Reference Standard
Supelco
Myristic acid, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Palmitic acid, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Palmitic acid, certified reference material, TraceCERT®
Sigma-Aldrich
Palmitoleic acid, ≥98.5% (GC), liquid
Sigma-Aldrich
Lignoceric acid, ≥99% (GC)
Sigma-Aldrich
γ-Linolenic acid, ≥99%, liquid
Sigma-Aldrich
Myristic acid, Sigma Grade, ≥99%
Sigma-Aldrich
Linolenic acid, ≥99%
Sigma-Aldrich
Palmitic acid, ≥95%, FCC, FG
Sigma-Aldrich
Palmitic acid, ≥98% palmitic acid basis (GC)
Sigma-Aldrich
Myristic acid, ≥98.0% (GC)
Supelco
Myristic acid, analytical standard
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Linolenic acid, analytical standard
Sigma-Aldrich
Linoleic acid, technical, 58-74% (GC)
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Palmitic acid, BioXtra, ≥99%
Sigma-Aldrich
Linoleic acid, ≥99%
Sigma-Aldrich
Palmitic acid, ≥99%
Sigma-Aldrich
Linoleic acid, liquid, BioReagent, suitable for cell culture
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Myristic acid, natural, ≥98.5%, FG
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Myristic acid, ≥95%, FCC, FG
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Linolenic acid, ~70% (GC)
Supelco
γ-Linolenic acid, analytical standard
Supelco
Palmitic acid, analytical standard
Supelco
Linoleic acid, analytical standard
Supelco
Palmitoleic acid, analytical standard
Palmitic acid, European Pharmacopoeia (EP) Reference Standard
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Palmitic acid, natural, 98%, FG
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Nervonic acid, ≥99% (capillary GC)
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Melting Point Standard 69-71°C, analytical standard