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  • Lipopolysaccharide enhanced renal vascular response to endothelin-1 through ETA overexpression in portal hypertensive rats.

Lipopolysaccharide enhanced renal vascular response to endothelin-1 through ETA overexpression in portal hypertensive rats.

Journal of gastroenterology and hepatology (2014-07-06)
Chiao-Lin Chuang, Hui-Chun Huang, Ching-Chih Chang, Fa-Yauh Lee, Jaw-Ching Wu, Jing-Yi Lee, Hsian-Guey Hsieh, Shou-Dong Lee

Hypo-perfusion resulting from intense renal vasoconstriction is traditionally contributed to renal dysfunction in advanced liver disease, although cumulative studies demonstrated renal vasodilatation with impaired vascular contractility to endogenous vasoconstrictors in portal hypertension and compensated liver cirrhosis. The pathophysiology of altered renal hemodynamics remains unclear. This study, using a rat model of portal hypertension with superimposed endotoxemia, was designed to delineate the evolution of renal vascular reactivity and vaso-regulatory gene expression during liver disease progression. Rats were randomized into sham surgery (SHAM) or partial portal vein ligation (PVL). Endotoxemia was induced by intraperitoneal injection of lipopolysaccharide (LPS) on the seventh day following surgery. Isolated kidney perfusion was performed at 0.5 h or 5 h after LPS to evaluate renal vascular response to endothelin-1. In contrast to impaired vascular contractility of SHAM rats, PVL rats displayed enhanced renal vascular reactivity to endothelin-1 at 5 h following endotoxemia. There were extensive upregulations of inducible nitric oxide synthase in kidney tissues of endotoxemic rats. The changes of renal endothelin receptor type A (ETA ) level paralleled with the changes of renal vascular reactivity in LPS-treated rats. Compared with SHAM rats, PVL rats showed increased renal ETA and phosphorylated extracellular-signal-regulated kinases 1/2 (p-ERK1/2) at 5 h after LPS. LPS-induced systemic hypotension induces a paradoxical change of renal vascular response to endothelin-1 between SHAM and PVL rats. LPS-induced renal vascular hyperreactivity in PVL rats was associated with upregulation of renal ETA and subsequent activation of ERK1/2 signaling.

Product Number
Product Description

Endothelin 1, ≥97% (HPLC), powder
Aucubin, primary reference standard
Ethanolamine, purified by redistillation, ≥99.5%
Ethanolamine, ACS reagent, ≥99.0%
Ethanolamine, puriss. p.a., ACS reagent, ≥99.0% (GC/NT)
Ethanolamine, analytical standard
Ethanolamine, ≥99%
Aucubin, analytical standard
Ethanolamine, liquid, BioReagent, suitable for cell culture, ≥98%
Ethanolamine, ≥98%
Trolamine impurity A, European Pharmacopoeia (EP) Reference Standard