• Home
  • Search Results
  • The effect of progesterone on systemic inflammation and oxidative stress in the rat model of sepsis.

The effect of progesterone on systemic inflammation and oxidative stress in the rat model of sepsis.

Indian journal of pharmacology (2014-12-30)
Ayse Nur Aksoy, Aysun Toker, Muhammet Celık, Mehmet Aksoy, Zekai Halıcı, Hulya Aksoy
ABSTRACT

To explore the protective effect of progesterone on inflammation and oxidative stress in a rat model of sepsis created by cecal ligation and puncture (CLP). Rats were randomly divided into 4 groups: Overiectomy group (OVX), sham operated (control), sepsis (CLP) group and progesterone-treated CLP group (CLP+ progesterone). The rats in CLP+ progesterone group received intraperitoneal progesterone (2 mg/kg). Cardiac blood samples were obtained for the measurement levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Tissue samples, including liver, kidney and uterus of rats were prepared to determine activities of myeloperoxidase (MPO), glutathione peroxidase (GPx) and levels of malondialdehyde (MDA). Increased serum IL-6 and TNF-α levels were found in the CLP group in comparison with the control group (P = 0.01, P = 0.02; respectively). In CLP+ progesterone group, mean MDA concentration of kidney tissue was significantly lower than in CLP group (P = 0.003). Liver MDA concentration of the CLP+ progesterone group was not significantly different from that of the control group. While there were no significant differences among groups regarding liver MPO; in the CLP group, MPO activity in kidney (P = 0.02) and uterine tissues (P = 0.03) were found to be significantly higher compared to the control group. In CLP+ progesterone group, mean MPO activities of all tissues were not different than those of control group. The uterine tissue GPx activity in the CLP+ progesterone group was not statistically significantly different from control group. We suggest that progesterone ameliorates sepsis syndrome by reduction of the inflammatory cytokines IL-6 and TNF-α, and by restoration of antioxidant enzyme activities in some tissues.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥98%
Sigma-Aldrich
Potassium phosphate tribasic, reagent grade, ≥98%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, for molecular biology, ≥99%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, BioXtra, ≥99%
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, BioUltra, for molecular biology, ≥99.0% (AT)
Sigma-Aldrich
Hexadecyltrimethylammonium bromide, ≥96.0% (AT)
Supelco
Hexadecyltrimethylammonium bromide, suitable for ion pair chromatography, LiChropur
USP
Cetrimonium bromide, United States Pharmacopeia (USP) Reference Standard
Supelco
Hexadecyltrimethylammonium bromide, analytical standard
SAFC
Hexadecyltrimethylammonium bromide