MilliporeSigma
  • Home
  • Search Results
  • Immobilization of Candida rugosa lipase onto an eco-friendly support in the presence of ionic liquid.

Immobilization of Candida rugosa lipase onto an eco-friendly support in the presence of ionic liquid.

Bioprocess and biosystems engineering (2014-11-14)
Rebeca Y Cabrera-Padilla, Milena C Lisboa, Matheus M Pereira, Renan T Figueiredo, Elton Franceschi, Alini T Fricks, Álvaro S Lima, Daniel P Silva, Cleide M F Soares
ABSTRACT

Candida rugosa lipase (CRL) was immobilized on an eco-friendly support poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV), by physical adsorption, using different ionic liquids (ILs) as immobilization additives. This was to investigate the influence of cationic core ([C4mpy]Cl, [C4min]Cl), of anions ([C4min]Cl, [C4min]N(CN)2, [C4min]Tf2N), and of cation chain length ([C2min]Tf2N, [C4min]Tf2N) in the immobilization process. The immobilized biocatalysts (IB) were characterized with respect to the morphological, physico-chemical properties, total activity recovery yield (Ya), and biochemical properties of more efficient IB were evaluated. Initially, it was found that the change of cationic core did not influence in Ya compared to the control. With change of anions, it was seen that the best result was obtained for the more hydrophobic anion (Tf2N), and finally increasing the cation chain length increased Ya. IB most efficient with [C4min]Tf2N obtained 78 % of Ya, more than twice the control value (30 %) and a considerable enhancement of operational stability compared with the control.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pyridine, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, HPLC/spectrophotometric grade
Sigma-Aldrich
Hexane, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99% (GC)
Sigma-Aldrich
Pyridine, ReagentPlus®, ≥99%
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, meets USP testing specifications
Sigma-Aldrich
Pyridine, biotech. grade, ≥99.9%
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, for molecular biology
Sigma-Aldrich
Pyridine, ACS reagent, ≥99.0%
Sigma-Aldrich
Hexane, suitable for HPLC, ≥97.0% (GC)
Sigma-Aldrich
Hexane, suitable for HPLC, ≥95%
Sigma-Aldrich
Hexane, HPLC Plus, for HPLC, GC, and residue analysis, ≥95%
Sigma-Aldrich
Pyridine, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Hexane, ReagentPlus®, ≥99%
Sigma-Aldrich
Pyridine, ACS reagent, ≥99.0%
Sigma-Aldrich
Pyridine, ReagentPlus®, ≥99%
Sigma-Aldrich
Ethanol, ACS reagent, prima fine spirit, without additive, F15 o1
Sigma-Aldrich
Hexane, Laboratory Reagent, ≥95%
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, for molecular biology
Supelco
Acetone solution, contains 20.0 % (v/v) acetonitrile, suitable for HPLC
Supelco
Acetone solution, certified reference material, 2000 μg/mL in methanol: water (9:1)
Sigma-Aldrich
Pyridine, anhydrous, 99.8%
Supelco
Hexane, suitable for HPLC, ≥95%
Sigma-Aldrich
Hexane, HPLC Plus, for HPLC, GC, and residue analysis, ≥95%
Sigma-Aldrich
Hexane, anhydrous, 95%
Supelco
Pyridine, analytical standard
Supelco
Hexane, analytical standard
Sigma-Aldrich
Pyridine, ≥99%
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, meets USP testing specifications