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Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.

Carbohydrate polymers (2014-05-13)
Zhen-Bin Wang, Juan-Juan Pei, Hai-Le Ma, Pan-Fu Cai, Jing-Kun Yan
ABSTRACT

Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively. PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents. PL-W and PL-A were mainly composed of glucose with high and low Mw fractions in various ratios. All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner. The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities. The Mws, monosaccharide compositions, and chemical structures of the polysaccharides also affected their antioxidant activities. PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.

MATERIALS
Product Number
Brand
Product Description

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SAFC
Galactose, plant-derived
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Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2O
Xylose, European Pharmacopoeia (EP) Reference Standard
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Hydrogen peroxide solution, contains inhibitor, 35 wt. % in H2O
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Hydrogen peroxide solution, 50 wt. % in H2O, stabilized
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Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H2O, meets USP testing specifications
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Hydrogen peroxide solution, purum p.a., ≥35% (RT)
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Hydrogen Peroxide Solution, 30% (w/w), puriss. p.a., reag. ISO, reag. Ph. Eur.
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D-(+)-Mannose, synthetic, ≥99%
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Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H2O, ACS reagent
USP
Galactose, United States Pharmacopeia (USP) Reference Standard
Supelco
Galactose, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis
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D-(+)-Mannose, wood, ≥99%
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D-(+)-Galactose, powder, anhydrous, BioReagent, suitable for cell culture, suitable for insect cell culture
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D-(+)-Galactose, ≥98%
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D-(+)-Galactose, BioXtra, pH 5.0-7.0 (20 °C, 1 M in H2O), ≥99%
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D-(+)-Galactose, ≥99%
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D-(+)-Glucose, ≥99.5% (GC)
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D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)
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D-(+)-Glucose, ACS reagent
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D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
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Hydrogen peroxide solution, 30 % (w/w) in H2O, contains stabilizer
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D-(+)-Glucose, BioXtra, ≥99.5% (GC)
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D-(+)-Glucose, Hybri-Max, powder, BioReagent, suitable for hybridoma
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D-(−)-Arabinose, ≥98%
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