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Rosmarinic acid mediated neuroprotective effects against H2O2-induced neuronal cell damage in N2A cells.

Life sciences (2014-07-25)
Hadi Ghaffari, M Venkataramana, Behrouz Jalali Ghassam, S Chandra Nayaka, A Nataraju, N P Geetha, H S Prakash

Oxidative stress plays a key role in several ailments including neurodegenerative conditions. The aim of the study was to demonstrate the effect of rosmarinic acid (RA) in preventing oxidative stress related death of neuronal cell lines. In the present study, we demonstrated direct neuroprotective effect of RA using H2O2-induced oxidative challenge in N2A mouse neuroblastoma cells. The mechanism of neutralization of H2O2-induced toxicity by RA was evaluated using MTT, lactate dehydrogenase, mitochondrial membrane potential (MMP), intracellular ROS, and comet assays. Up-regulation of brain neuronal markers at molecular level was performed by RT-PCR. Results presented in the paper indicate that H2O2-induced cytotoxicity in N2A cells was suppressed by treatment with RA. Moreover, RA is very effective in attenuating the disruption of lactate dehydrogenase, mitochondrial membrane potential and intracellular ROS. Pretreatment with RA significantly prevents genotoxicity (3.7-fold, p<0.01) and promotes the up-regulation of tyrosine hydroxylase (TH) (4.5-fold, p<0.01), and brain-derived neurotrophic factor (BDNF) genes (5.4-fold, p<0.01) against H2O2-induced cytotoxicity in N2A cells. Our results revealed that N2A cells are suitable cellular models to evaluate neuroprotective effects of RA, and suggest that RA may potentially serve as an agent for prevention of several human neurodegenerative diseases caused by oxidative stress.

Product Number
Product Description

Rhodamine 123, mitochondrial specific fluorescent dye
Rhodamine 123, BioReagent, for fluorescence, ≥85% (HPLC)
Sodium pyruvate, anhydrous, free-flowing, Redi-Dri, ReagentPlus®, ≥99%
Rosmarinic acid, 96%
Sodium pyruvate, powder, BioXtra, suitable for mouse embryo cell culture
Sodium pyruvate, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
Sodium pyruvate, ReagentPlus®, ≥99%
Sodium pyruvate, Hybri-Max, powder, suitable for hybridoma
Sodium pyruvate, BioXtra, ≥99%
Rosmarinic acid, primary reference standard
Rosmarinic acid, ≥98% (HPLC), from Rosemarinus officinalis L.
Rosmarinic acid, European Pharmacopoeia (EP) Reference Standard