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Attenuation of lung cancer stem cell tumorigenesis and metastasis by cisplatin.

Experimental lung research (2014-08-26)
Shenxu Wang, Sai Ma, Xiujuan Li, Zengfu Xue, Xiaotian Zhang, Weiwei Fan, Yongzhan Nie, Kaichun Wu, Xiaoyuan Chen, Feng Cao
ABSTRACT

To investigate the effect of cisplatin on the growth and metastasis abilities of lung cancer stem cells (CSCs) via molecular imaging. The expression changes of lung CSCs cell marker in A549-Luc-C8 human non-small-cell lung cancer (NSCLC) cell line with or without cisplatin treatment were detected by flow cytometry. The tumorigenesis and metastasis abilities of A549-Luc-C8 cells were monitored both in vitro and in vivo, and the mechanism was assessed by gene sequencing. About 1%-2% of CSCs were detected in A549-Luc-C8 cells and decreased CSCs percentage was observed after cisplatin treatment. Attenuated tumorigenesis and metastasis abilities of A549-Luc-C8 cells were found in cisplatin treated group. Decreased percentage of CSCs in A549-Luc-C8 cells can be induced by cisplatin treatment, which may partly be attributed to the attenuated expression of growth factors.

MATERIALS
Product Number
Brand
Product Description

Cisplatin, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
FGF-2 human, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
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trans-Platinum(II)diammine dichloride
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FGF-2 human, recombinant, expressed in insect cells, ≥85% (SDS-PAGE)
SAFC
BIS-TRIS
SAFC
BIS-TRIS
Cisplatin impurity A, European Pharmacopoeia (EP) Reference Standard
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BIS-TRIS, BioXtra, ≥98.0% (titration)
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BIS-TRIS, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%
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USP
Transplatin, United States Pharmacopeia (USP) Reference Standard
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cis-Diamineplatinum(II) dichloride, ≥99.9% trace metals basis