• Home
  • Search Results
  • Mesenchymal stem cells promote cardiac muscle repair via enhanced neovascularization.

Mesenchymal stem cells promote cardiac muscle repair via enhanced neovascularization.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2015-03-15)
Jianfeng Zhang, Yicheng Wu, Anqing Chen, Qiang Zhao
ABSTRACT

Transplantation of bone-marrow derived stem/progenitor cells has an established therapeutic effect on cardiac muscle repair after injury. However, the exact mechanism that underlies this phenomenon is not completely understood. Here we transplanted mesenchymal stem cells (MSCs), a major population from the bone-marrow derived stem/progenitor cells, and studied its effects on cardiac muscle repair after injury. MSCs transplantation significantly improved cardiac muscle repair after injury. The grafted MSCs did not significantly differentiate into cardiac muscle cells themselves, but appeared to induce neovascularization in the injured heart. In a loss-of-function experiment, we further show that production and secretion of placental growth factor, but not vascular endothelial growth factor A in MSCs, were essential for the MSCs-induced neovasularization after cardiac muscle injury to facilitate cardiac muscle repair. Our study thus sheds light on an undescribed role of placental growth factor in cardiac muscle regeneration.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
Dimethyl sulfoxide, for molecular biology
Sigma-Aldrich
Dimethyl sulfoxide, anhydrous, ≥99.9%
Sigma-Aldrich
Dimethyl sulfoxide, ACS reagent, ≥99.9%
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
Vitamin D3 solution, 100 μg/mL in ethanol, 97% (CP)
Sigma-Aldrich
Dexamethasone, powder, BioReagent, suitable for cell culture, ≥97%
Sigma-Aldrich
Dimethyl sulfoxide, suitable for HPLC, ≥99.7%
Sigma-Aldrich
Dimethyl sulfoxide, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
Dexamethasone, ≥98% (HPLC), powder
Sigma-Aldrich
Cholecalciferol, ≥98% (HPLC)
Supelco
Cholecalciferol (Vitamin D3), Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)
Sigma-Aldrich
Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, BioReagent, suitable for fluorescence, ≥90% (HPLC)
Supelco
Vitamin D3 solution, 1 mg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®
Supelco
Dexamethasone, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Dexamethasone, powder, γ-irradiated, BioXtra, suitable for cell culture, ≥80% (HPLC)
Sigma-Aldrich
Dimethyl sulfoxide, PCR Reagent
Supelco
Cholecalciferol (D3), analytical standard
Sigma-Aldrich
Bicinchoninic acid disodium salt hydrate, ≥98% (HPLC)
Sigma-Aldrich
Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water
Sigma-Aldrich
Xylazine, ≥99%
USP
Cholecalciferol, United States Pharmacopeia (USP) Reference Standard
Cholecalciferol, European Pharmacopoeia (EP) Reference Standard
Cholecalciferol for system suitability, European Pharmacopoeia (EP) Reference Standard
Supelco
Dimethyl sulfoxide, analytical standard
Sigma-Aldrich
Cholecalciferol, meets USP testing specifications