MilliporeSigma
  • Home
  • Search Results
  • Novel and simple headspace in-tube microextraction coupled with capillary electrophoresis.

Novel and simple headspace in-tube microextraction coupled with capillary electrophoresis.

Journal of chromatography. A (2014-05-09)
Hye Ryeo Lee, Sung Min Cho, Jihye Kim, Doo Soo Chung
ABSTRACT

In liquid phase microextraction, high enrichment factors can be obtained using an acceptor phase of small volume. By hanging an acceptor drop at the separation capillary tip, single drop microextraction (SDME) can be in-line coupled with capillary electrophoresis (CE). The small surface-to-volume ratio of the drop enables high enrichment factors to be obtained in a short time. One practical issue in SDME is how to keep the drop attached to the capillary stable. Here, we present novel but extremely simple in-tube microextraction (ITME) using the liquid inside the capillary as an acceptor phase, without forming a drop at the capillary tip. As a first example, ITME has been combined with headspace (HS) extraction. Simply by placing a capillary filled with a basic run buffer in the HS above an acidic donor solution, volatile acidic analytes were extracted into the acceptor phase in the capillary. After extraction, electrophoresis of the extracts in the capillary was carried out. Owing to the robust nature of the acceptor phase, the extraction temperature and time ranges of HS-ITME can be extended significantly, compared to HS-SDME. The enrichment factors for chlorophenols in a standard solution were up to 1100 under an optimal HS-ITME condition of 80°C for 15min and the limits of detections (LODs) obtained by monitoring the absorbance at 214nm were about 4nM. The whole procedures of HS-ITME-CE were carried out automatically using built-in programs of a commercial CE instrument.

MATERIALS
Product Number
Brand
Product Description

Supelco
2,6-Dichlorophenol, PESTANAL®, analytical standard
Sigma-Aldrich
Boric acid-11B, ≥99 atom % 11B
Sigma-Aldrich
2,6-Dichlorophenol, 99%
Supelco
2,3,6-Trichlorophenol, PESTANAL®, analytical standard
Sigma-Aldrich
Boric acid, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Sodium tetraborate decahydrate, puriss. p.a., ACS reagent, reag. ISO, buffer substance, ≥99.5%
Sigma-Aldrich
Sodium tetraborate decahydrate, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Sodium tetraborate decahydrate, ACS reagent, ≥99.5%
Sigma-Aldrich
Sodium tetraborate decahydrate, puriss., meets analytical specification of Ph. Eur., BP, NF, 99.0-103.0%
Sigma-Aldrich
Boric acid, ACS reagent, ≥99.5%
Sigma-Aldrich
Boric acid, 99.999% trace metals basis
Sigma-Aldrich
Boric acid, tablet, 1 g boric acid per tablet
Sigma-Aldrich
Sodium tetraborate decahydrate, BioXtra, ≥99.5%
Sigma-Aldrich
Boric acid, suitable for electrophoresis, ≥99.5%
Sigma-Aldrich
Boric acid, BioReagent, for molecular biology, suitable for cell culture, suitable for plant cell culture, ≥99.5%
Sigma-Aldrich
Boric acid, BioXtra, ≥99.5%
Supelco
Benalaxyl-M, PESTANAL®, analytical standard
Sigma-Aldrich
Boric acid, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., buffer substance, ≥99.8%
Sigma-Aldrich
Boric acid, puriss., meets analytical specification of Ph. Eur., BP, NF, 99.5-100.5%, powder
Sigma-Aldrich
Boric acid, BioUltra, for molecular biology, ≥99.5% (T)
Sigma-Aldrich
Boric acid, 99.97% trace metals basis