• Home
  • Search Results
  • Comparison of phytoncide with sirolimus as a novel drug candidate for drug-eluting stent.

Comparison of phytoncide with sirolimus as a novel drug candidate for drug-eluting stent.

Biomaterials (2015-01-27)
Sung Nam Kang, Si-Eun Kim, Jiyeon Choi, Kwideok Park, Jae Hwan Goo, Doo Sun Sim, Young Joon Hong, Ju Han Kim, Yoon Ki Joung, Jay Lee, Myung Ho Jeong, Dong Keun Han
ABSTRACT

A drug-eluting stent (DES) is one of the commonly used treatment techniques in percutaneous coronary intervention (PCI). Sirolimus (SRL) has been widely used for DES as a drug for suppressing neointimal hyperplasia causing restenosis. Phytoncides (PTC) are compounds released from trees and plants, and their solutions contain monoterpenoids such as α-pinene, careen, and myrceen. Some studies have reported that these components exhibit antioxidant, antimicrobial, and anti-inflammatory activities. We hypothesized that PTC may become an alternative drug to SRL for DES, exhibiting alleviated side effects as compared to SRL. A PTC-incorporated stent was compared with an SRL-incorporated stent in terms of physicochemical, pharmacokinetic, and biological properties. In in vitro studies, the effects of each drug on cells were investigated. The results showed that both drugs exhibited similar cytotoxicity, anti-inflammation, and antiproliferation effects. However, these effects resulted from different mechanisms associated with cells, as seen in the immunofluorescence result. An in vivo assay showed that the lumen area was significantly larger and the neointimal area was significantly smaller in SRL- and PTC-loaded stents compared to a drug-unloaded stent. These results suggest that phytoncide can be a feasible alternative drug to SRL for advanced DES although more studies are needed.

MATERIALS
Product Number
Brand
Product Description

Ascorbic acid, European Pharmacopoeia (EP) Reference Standard
USP
Residual Solvent Class 2 - Acetonitrile, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Methanol solution, (Methanol:Dimethyl sulfoxide 1:1 (v/v))
Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
Supelco
Residual Solvent - Chloroform, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
Supelco
Dichloromethane solution, contains 10 % (v/v) methanol
USP
Ascorbic acid, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
Supelco
Methanol solution, contains 0.1 % (v/v) trifluoroacetic acid, 5 % (v/v) water, suitable for HPLC
Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
Supelco
Ascorbic Acid, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Acetonitrile, HPLC Plus, ≥99.9%, poly-coated bottles
Sigma-Aldrich
Acetonitrile, ≥99.5%, ACS reagent
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
Sigma-Aldrich
Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer
Supelco
Acetonitrile, analytical standard
Supelco
Dichloromethane solution, certified reference material, 200 μg/mL in methanol
Supelco
Dichloromethane, analytical standard, stabilized
Sigma-Aldrich
Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer
Supelco
Dichloromethane, ≥99.9%
Supelco
Dichloromethane solution, certified reference material, 5000 μg/mL in methanol
Supelco
Acetonitrile, HPLC grade, ≥99.93%
Supelco
Dichloromethane, Selectophore, ≥99.5%
Supelco
Dichloromethane, analytical standard
Hydrocortisone for peak identification, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Acetonitrile
Supelco
Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Methylene Chloride, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Dichloromethane, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer