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  • The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.

The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.

Nature (2014-06-05)
Baris Bingol, Joy S Tea, Lilian Phu, Mike Reichelt, Corey E Bakalarski, Qinghua Song, Oded Foreman, Donald S Kirkpatrick, Morgan Sheng
ABSTRACT

Cells maintain healthy mitochondria by degrading damaged mitochondria through mitophagy; defective mitophagy is linked to Parkinson's disease. Here we report that USP30, a deubiquitinase localized to mitochondria, antagonizes mitophagy driven by the ubiquitin ligase parkin (also known as PARK2) and protein kinase PINK1, which are encoded by two genes associated with Parkinson's disease. Parkin ubiquitinates and tags damaged mitochondria for clearance. Overexpression of USP30 removes ubiquitin attached by parkin onto damaged mitochondria and blocks parkin's ability to drive mitophagy, whereas reducing USP30 activity enhances mitochondrial degradation in neurons. Global ubiquitination site profiling identified multiple mitochondrial substrates oppositely regulated by parkin and USP30. Knockdown of USP30 rescues the defective mitophagy caused by pathogenic mutations in parkin and improves mitochondrial integrity in parkin- or PINK1-deficient flies. Knockdown of USP30 in dopaminergic neurons protects flies against paraquat toxicity in vivo, ameliorating defects in dopamine levels, motor function and organismal survival. Thus USP30 inhibition is potentially beneficial for Parkinson's disease by promoting mitochondrial clearance and quality control.

MATERIALS
Product Number
Brand
Product Description

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Monoclonal Anti-TIMM8A antibody produced in mouse, clone 2F11, purified immunoglobulin, buffered aqueous solution
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Anti-USP30 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
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Anti-RHOT1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution