MilliporeSigma

Doxycycline administration improves fascial interface in hernia repair.

The Journal of surgical research (2014-06-16)
Job C Tharappel, Curtis E Bower, Jennifer Whittington Harris, Sandeep K Ramineni, David A Puleo, J Scott Roth
ABSTRACT

Despite improvements in ventral hernia repair techniques, their recurrence rates are unacceptably high. Increased levels of matrix metalloproteinases (MMPs) and reduced collagen-1 to -3 ratios are implicated in incisional hernia formation. We have recently shown doxycycline treatment for 4 wk after hernia repair reduced MMP levels, significantly increased collagen-1 to -3 ratios, and increased tensile strength of repaired interface fascia. However, this increase was not statistically significant. In this study, we extended treatment duration to determine whether this would impact the tensile strength of the repaired interface fascia. Thirty-two male Sprague-Dawley rats underwent incision hernia creation and subsequent repair with polypropylene mesh. The animals received either saline (n = 16) or doxycycline (n = 16) beginning from 1 day before hernia repair until the end of survival time of 6 wk (n = 16) or 12 wk (n = 16). Tissue samples were investigated for MMPs and collagen subtypes using Western blot procedures, and tensiometric analysis was performed. At both 6 and 12 wk after hernia repair, the tensiometric strength of doxycycline-treated mesh to fascia interface (MFI) tissue showed a statistically significant increase when compared with untreated control MFI. In both groups, collagen-1, -2, and -3 ratios were remarkably increased in doxycycline-treated MFI. At 6 wk, the doxycycline-treated MFI group showed a significant decrease in MMP-2, an increase in MMP-3, and no change in MMP-9. At 12 wk, MMP-9 showed a remarkable reduction, whereas MMP-2 and -3 protein levels increased in the doxycycline-treated MFI group. Doxycycline administration results in significantly improved strength of repaired fascial interface tissue along with a remarkable increase in collagen-1, -2, and -3 ratios.

MATERIALS
Product Number
Brand
Product Description

Supelco
Doxycycline hyclate, VETRANAL®, analytical standard
Sigma-Aldrich
Doxycycline hyclate
Doxycycline hyclate, European Pharmacopoeia (EP) Reference Standard
Supelco
Doxycycline hyclate, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Doxycycline hyclate, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Endoproteinase Arg-C from mouse submaxillary gland, suitable for protein sequencing, lyophilized powder
Sigma-Aldrich
Doxycycline monohydrate