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  • In vivo measurement of extravasation of silver nanoparticles into liver extracellular space by push-pull-based continuous monitoring system.

In vivo measurement of extravasation of silver nanoparticles into liver extracellular space by push-pull-based continuous monitoring system.

Toxicology letters (2014-04-08)
Cheng-Kuan Su, Ching-Wen Hung, Yuh-Chang Sun
ABSTRACT

With the increasing prevalence of silver nanoparticles (AgNPs) in various products, whether such AgNPs will introduce new injury mechanisms from new pathologies remains to be determined. From the toxicokinetic viewpoint, it is vital to have in-depth knowledge of their in vivo transport kinetics and extravasation phenomenon. By combining push-pull perfusion sampling, in-tube solid phase extraction, and inductively coupled plasma mass spectrometry, we used an in vivo push-pull-based continuous monitoring system to investigate in vivo transport kinetics of extracellular AgNPs in living rat liver with a detection limit and temporal resolution of 0.64μgL(-1) and 10min, respectively. Before administration into living rats, the pre-incubation in DMEM with 10% FBS for 8h was adopted as the optimized exposure condition for the used AgNPs. After repeated-dose treatments, we observed a higher concentration of AgNPs in the liver extracellular space, suggesting that AgNP clearance by the reticuloendothelial system (RES) may be blocked by a prior administration of AgNPs. Future studies on AgNP distribution in different liver compartments (blood stream, extracellular space and Kupffer cells/hepatocytes) are necessary for defining the risks and benefits of AgNP applications.

MATERIALS
Product Number
Brand
Product Description

Silver, foil, 10mm disks, thickness 0.02mm, 99.97%
Silver, foil, 10mm disks, thickness 0.004mm, 99.97%
Silver, foil, 10mm disks, thickness 0.20mm, as rolled, 99.95+%
Silver, foil, 10mm disks, thickness 0.025mm, as rolled, 99.95+%
Silver, foil, 10mm disks, thickness 0.125mm, as rolled, 99.99+%
Silver, foil, 10mm disks, thickness 1.0mm, as rolled, 99.99+%
Silver, foil, 10mm disks, thickness 0.025mm, as rolled, 99.99+%
Silver, foil, not light tested, 25x25mm, thickness 0.00075mm, temporary acrylic support, 99.97%
Silver, insulated wire, 1m, conductor diameter 0.075mm, insulation thickness 0.010mm, nylon insulation, 99.99%
Silver, insulated wire, 2m, conductor diameter 0.25mm, insulation thickness 0.015mm, epoxy insulation, 99.99%
Silver, insulated wire, 10m, conductor diameter 0.075mm, insulation thickness 0.010mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Silver, insulated wire, 2m, conductor diameter 0.125mm, insulation thickness 0.0125mm, polyester insulation, 99.99%
Silver, insulated wire, 10m, conductor diameter 0.125mm, insulation thickness 0.015mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Silver, insulated wire, 20m, conductor diameter 0.075mm, insulation thickness 0.010mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Silver, insulated wire, 2m, conductor diameter 0.075mm, insulation thickness 0.010mm, nylon insulation, 99.99%
Silver, insulated wire, 10m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyurethane insulation, 99.99%
Silver, microfoil, disks, 25mm, thinness 0.5μm, specific density 522.6μg/cm2, permanent mylar 3.5μm support, 99.95+%
Silver, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.0075mm, polyester insulation, 99.99%
Silver, insulated wire, 50m, conductor diameter 0.05mm, insulation thickness 0.0075mm, polyester insulation, 99.99%
Silver, insulated wire, 10m, conductor diameter 0.25mm, insulation thickness 0.024mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Silver, insulated wire, 5m, conductor diameter 0.125mm, insulation thickness 0.0125mm, polyester insulation, 99.99%
Silver, insulated wire, 2m, conductor diameter 0.25mm, insulation thickness 0.005mm, polyurethane insulation, 99.99%
Silver, insulated wire, 2m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
Silver, insulated wire, 50m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyurethane insulation, 99.99%
Silver, insulated wire, 20m, conductor diameter 0.125mm, insulation thickness 0.0125mm, polyimide insulation, 99.9%
Silver, microfoil, disks, 10mm, thinness 1.0μm, specific density 1098μg/cm2, permanent mylar 3.5μm support, 99.95%
Silver, insulated wire, 10m, conductor diameter 0.075mm, insulation thickness 0.010mm, nylon insulation, 99.99%
Silver, insulated wire, 50m, conductor diameter 0.25mm, insulation thickness 0.015mm, epoxy insulation, 99.99%
Silver, insulated wire, 2m, conductor diameter 0.25mm, insulation thickness 0.024mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Silver, insulated wire, 2m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyurethane insulation, 99.99%