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  • Early specification of CD8+ T lymphocyte fates during adaptive immunity revealed by single-cell gene-expression analyses.

Early specification of CD8+ T lymphocyte fates during adaptive immunity revealed by single-cell gene-expression analyses.

Nature immunology (2014-03-04)
Janilyn Arsenio, Boyko Kakaradov, Patrick J Metz, Stephanie H Kim, Gene W Yeo, John T Chang
ABSTRACT

T lymphocytes responding to microbial infection give rise to effector cells that mediate acute host defense and memory cells that provide long-lived immunity, but the fundamental question of when and how these cells arise remains unresolved. Here we combined single-cell gene-expression analyses with 'machine-learning' approaches to trace the transcriptional 'roadmap' of individual CD8(+) T lymphocytes throughout the course of an immune response in vivo. Gene-expression signatures predictive of eventual fates could be discerned as early as the first T lymphocyte division and may have been influenced by asymmetric partitioning of the receptor for interleukin 2 (IL-2Rα) during mitosis. Our findings emphasize the importance of single-cell analyses in understanding fate determination and provide new insights into the specification of divergent lymphocyte fates early during an immune response to microbial infection.

MATERIALS
Product Number
Brand
Product Description

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Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)
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Albumin from chicken egg white, For use as a marker in SDS-PAGE
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Albumin from chicken egg white, lyophilized powder, ≥90% (agarose gel electrophoresis)
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Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)