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- Evaluation of cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate, and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme for recovery of Clostridium difficile isolates from fecal samples.
Evaluation of cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate, and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme for recovery of Clostridium difficile isolates from fecal samples.
Journal of clinical microbiology (2013-06-28)
Kerin L Tyrrell, Diane M Citron, Eliza S Leoncio, C Vreni Merriam, Ellie J C Goldstein
PMID23804392
ABSTRACT
Cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate (CCFA-HT), and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme (CCMB-TAL) were compared for recovery of Clostridium difficile from 120 stool specimens. Compared to CCFA, CCFA-HT enhanced C. difficile growth and improved recovery by 4%. In a separate study, 9% (8/91) of stool samples previously C. difficile negative on plate medium were C. difficile positive when cultured in CCMB-TAL.
MATERIALS
Product Number
Brand
Product Description
Mannitol, European Pharmacopoeia (EP) Reference Standard
Sodium taurocholate, BRP, European Pharmacopoeia (EP) Reference Standard
Cefoxitin sodium, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Agar, microbiology tested, suitable for plant cell culture, suitable for cell culture, powder
Sigma-Aldrich
Agar, Bacteriological, microbiology tested, suitable for plant cell culture, granular