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Artificial reductant enhancement of the Lowry method for protein determination.

Analytical biochemistry (1986-06-01)
E Larson, B Howlett, A Jagendorf
ABSTRACT

Addition of dithiothreitol in the Lowry procedure 3 min after adding the Folin-Ciocalteau reagent produces immediate color development, with 35 to 60% greater absorbance per mass of protein used.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Folin & Ciocalteu′s phenol reagent, suitable for determination of total protein by Lowry method, 2 N