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Cannabinoid photolabelling of a G-protein gamma-subunit in mouse peritoneal cells.

Life sciences (1995-01-01)
S H Burstein, M Debatis, A Subramanian
ABSTRACT

Binding and photoactivation of a cannabinoid-derived ligand to intact mouse peritoneal cells has resulted in the labelling of a G-protein gamma-subunit. The assignment of structure is based on the product's physical characteristics and its ability to react with a polyclonal antiserum raised against the partial amino acid sequence for a gamma-subunit expressed in spleen cells. The binding characteristics of the ligand to the cells suggests that this gamma-subunit, and its associated alpha and beta subunits, are located in close proximity to one of the transmembrane cannabinoid receptors. Our findings further suggest a possible experimental approach to identifying receptor-G-protein complexes in intact cells.

MATERIALS
Product Number
Brand
Product Description

Supelco
(−)-Δ8-THC solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®