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Biologic testing of leachable aromatic compounds from denture base materials.

Acta odontologica Scandinavica (1995-12-01)
H Lygre, G Moe, E Solheim, N R Gjerdet
ABSTRACT

The aromatic compounds phenyl benzoate (PB), phenyl salicylate (PS), and biphenyl (BP), which have previously been found to leach from poly(methyl methacrylate) denture base materials, were tested for cytotoxicity and biologic effects by L929 cells in culture. The octanol-water partition coefficient (log P(ow), a descriptor for the lipophilicity, was determined for the compounds. Cytotoxicity was evaluated by total cell growth and the plating efficiency test, and biologic effects by the total fatty acid composition of L929 cells. The commonly used tests, total cell growth and plating efficiency, did not show any significant changes of the cells due to the compounds. On the other hand, BP and PS, in particular, induced changes in the total fatty acid composition of L929 cells. The problem of bioavailability of aromatic compounds in cell culture assays and the relation to lipophilicity was addressed.

MATERIALS
Product Number
Brand
Product Description

Supelco
Mettler-Toledo Calibration substance ME 30034252, Phenyl salicylate, traceable to primary standards
Sigma-Aldrich
Biphenyl-4-carboxylic acid, 95%
Supelco
Phenyl salicylate melting point standard, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Phenyl salicylate, ≥99%, FG
Sigma-Aldrich
Phenyl salicylate, ReagentPlus®, 99%