MilliporeSigma
  • Home
  • Search Results
  • Isolation and characterization of a trypsin inhibitor from the seeds of kohlrabi (Brassica napus var. rapifera) belonging to the napin family of storage proteins.

Isolation and characterization of a trypsin inhibitor from the seeds of kohlrabi (Brassica napus var. rapifera) belonging to the napin family of storage proteins.

Carlsberg research communications (1989-01-01)
I B Svendsen, D Nicolova, I Goshev, N Genov
ABSTRACT

A trypsin inhibitor with a Km of 5 x 10(-5) M has been isolated from kohlrabi (Brassica napus var. rapifera). Subtilisin DY is inhibited only weakly and chymotrypsin not at all. The inhibitor is closely related to napin as determined by amino acid sequence analysis which also showed the inhibitor to be polymorphous. The inhibitor has been further characterized by means of molecular weight determination using SDS gel-electrophoresis and by amino acid analysis, fluorimetry as well as circular dichroism. A simplified method for purification of napins is given.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Trypsin Inhibitor, Defined (1X) Solution, Animal component free, BioReagent, suitable for cell culture
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), lyophilized powder, ~10000 U/mg
Sigma-Aldrich
Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), lyophilized powder
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), BioReagent, For use as a marker in SDS-PAGE
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), solution, sterile-filtered, suitable for, suitable for cell culture
Sigma-Aldrich
Trypsin inhibitor from chicken egg white, Type III-O (free of ovoinhibitor)
Sigma-Aldrich
Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), lyophilized powder
Sigma-Aldrich
Trypsin inhibitor from Glycine max (soybean), Isoelectric focusing marker, pI 4.6