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[Antimicrobial activity of stable silver nanoparticles of a certain size].

[Antimicrobial activity of stable silver nanoparticles of a certain size].

Prikladnaia biokhimiia i mikrobiologiia (2013-06-26)

Iu P Mukha, A M Eremenko, N P Smirnova, A I Mikhienkova, G I Korchak, V F Gorchev, A Iu Chunikhin

ABSTRACT

Conditions for obtaining stable silver nanoparticles smaller than 10 nm were developed using a binary stabilizer polyvinylpyrrolidone/sodium dodecylsulphate in optimal ratio. Optical spectra, morphology and dependence of size of the nanoparticles on the amount of reducing agent were studied. Colloidal solutions of nanosilver showed a high bactericidal activity against strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and fungicidal activity against Candida albicans. The mechanism of action of nanosized silver on microbial cell was examined by laser scanning confocal microscope using fluorescent label. First step of antimicrobial effect on microorganisms was membrane damage and penetration of silver nanoparticles into the cell. Prolonged stability of nanoparticles and their antimicrobial activity over the past two years were showed.

MATERIALS

Product Number

Brand

Product Description

327077

Sigma-Aldrich

Silver, flakes, 10 μm, ≥99.9% trace metals basis

327107

Sigma-Aldrich

Silver, powder, <45 μm, ≥99.99% trace metals basis

437190

Sigma-Aldrich

Polyvinylpyrrolidone, average M_w ~1,300,000 by LS

327093

Sigma-Aldrich

Silver, powder, 5-8 μm, ≥99.9% trace metals basis

P5288

Sigma-Aldrich

Polyvinylpyrrolidone, for molecular biology, nucleic acid hybridization tested, mol wt 360,000

P2307

Sigma-Aldrich

Polyvinylpyrrolidone, suitable for plant cell culture, average mol wt 10,000

PVP360

Sigma-Aldrich

Polyvinylpyrrolidone, mol wt (number average molecular weight Mn 360)

P0930

Sigma-Aldrich

Polyvinylpyrrolidone, powder, BioXtra, suitable for mouse embryo cell culture

PVP10

Sigma-Aldrich

Polyvinylpyrrolidone, average mol wt 10,000

PVP40

Sigma-Aldrich

Polyvinylpyrrolidone, average mol wt 40,000

327085

Sigma-Aldrich

Silver, powder, 2-3.5 μm, ≥99.9% trace metals basis

348775

Sigma-Aldrich

Silver, rod, diam. 3.2 mm, 99.98% trace metals basis

345075

Sigma-Aldrich

Silver, foil, thickness 0.5 mm, 99.9% trace metals basis

348759

Sigma-Aldrich

Silver, wire, diam. 1.5 mm, ≥99.99% trace metals basis

265543

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Silver, foil, thickness 1.0 mm, 99.9% trace metals basis

373249

Sigma-Aldrich

Silver, evaporation slug, diam. × L 0.6 cm × 1.2 cm, 99.99% trace metals basis

234257

Sigma-Aldrich

Polyvinylpyrrolidone, powder, average M_w ~29,000

71736

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H₂O

81420

Sigma-Aldrich

Polyvinylpyrrolidone, K 30

856568

Sigma-Aldrich

Polyvinylpyrrolidone, powder, average M_w ~55,000

576832

Sigma-Aldrich

Silver, nanopowder, <100 nm particle size, contains PVP as dispersant, 99.5% trace metals basis

81430

Sigma-Aldrich

Polyvinylpyrrolidone solution, K 60, 45% in H₂O

81440

Sigma-Aldrich

Polyvinylpyrrolidone, K 90

05030

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 20% in H₂O

90268

Sigma-Aldrich

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L4509

Sigma-Aldrich

Sodium dodecyl sulfate, ReagentPlus^®, ≥98.5% (GC)

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Sigma-Aldrich

Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

L3771

Sigma-Aldrich

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L6026

Sigma-Aldrich

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P2472

Sigma-Aldrich

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