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  • Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection.

Inflammatory Flt3l is essential to mobilize dendritic cells and for T cell responses during Plasmodium infection.

Nature medicine (2013-05-21)
Pierre Guermonprez, Julie Helft, Carla Claser, Stephanie Deroubaix, Henry Karanje, Anna Gazumyan, Guillaume Darasse-Jèze, Stephanie B Telerman, Gaëlle Breton, Heidi A Schreiber, Natalia Frias-Staheli, Eva Billerbeck, Marcus Dorner, Charles M Rice, Alexander Ploss, Florian Klein, Melissa Swiecki, Marco Colonna, Alice O Kamphorst, Matthew Meredith, Rachel Niec, Constantin Takacs, Fadi Mikhail, Aswin Hari, David Bosque, Tom Eisenreich, Miriam Merad, Yan Shi, Florent Ginhoux, Laurent Rénia, Britta C Urban, Michel C Nussenzweig
ABSTRACT

Innate sensing mechanisms trigger a variety of humoral and cellular events that are essential to adaptive immune responses. Here we describe an innate sensing pathway triggered by Plasmodium infection that regulates dendritic cell homeostasis and adaptive immunity through Flt3 ligand (Flt3l) release. Plasmodium-induced Flt3l release in mice requires Toll-like receptor (TLR) activation and type I interferon (IFN) production. We found that type I IFN supports the upregulation of xanthine dehydrogenase, which metabolizes the xanthine accumulating in infected erythrocytes to uric acid. Uric acid crystals trigger mast cells to release soluble Flt3l from a pre-synthesized membrane-associated precursor. During infection, Flt3l preferentially stimulates expansion of the CD8-α(+) dendritic cell subset or its BDCA3(+) human dendritic cell equivalent and has a substantial impact on the magnitude of T cell activation, mostly in the CD8(+) compartment. Our findings highlight a new mechanism that regulates dendritic cell homeostasis and T cell responses to infection.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Uric acid, ≥99%, crystalline
Sigma-Aldrich
Uric acid, BioXtra, ≥99% (HPLC)