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[Callus cultivation and determination of gentiopicroside from Gentiana macrophylla].

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (2012-12-12)
Qian Hou, Mei Guo, Fan Zhang, Jin-xia Zhou, Li-sha Liu
ABSTRACT

To explore and improve the utilization efficiency of Gentiana macrophylla resources. The germination of Gentiona macrophylla seeds were used as explants to induce calluses; Cultured on various culture media and condition for callus induction and subculture by test; The content of gentiopicroside in callus was determined by HPLC. The optimum medium for callus was MS +2,4-D (1.0 mg/L) + 6-BA (0.5 mg/L), and MS +2,4-D (1.0 mg/L) +6-BA (0.5 mg/L) + NAA (0.5 mg/L) was the optimum for subculture,the optimum time of subculture was 30 d; The accumulated gentiopicroside reached the highest level with 0.78 mg/g DW when entered 28-30 d. The optimized culture method of callus of Gentiana. macrophylla is obtained; The growth curve of callus of Gentiona macrophylla turns on "S" appearance and the accumulated gentiopicroside increases along with the callus appreciation.

MATERIALS
Product Number
Brand
Product Description

Supelco
2,4-D, certified reference material, TraceCERT®
Supelco
2,4-D, PESTANAL®, analytical standard
Supelco
2,4-D solution, certified reference material, 5000 μg/mL in acetonitrile
Supelco
2,4-D, PESTANAL®, analytical standard
Sigma-Aldrich
2,4-Dichlorophenoxyacetic acid, ≥95%, crystalline
Sigma-Aldrich
2,4-Dichlorophenoxyacetic acid, 97%