MilliporeSigma
  • Home
  • Search Results
  • Determination method for mono- and diethanolamine in workplace air by high-performance liquid chromatography.

Determination method for mono- and diethanolamine in workplace air by high-performance liquid chromatography.

Journal of occupational health (2012-07-21)
Akito Takeuchi, Takashi Kitade, Akira Jukurogi, Warren Hendricks, Yuichiro Kaifuku, Kenji Shibayama, Shuichiro Natsumeda, Hirokazu Ota, Shu Yamada, Kimiaki Sumino, Akira Namera, Seiichiro Kanno
ABSTRACT

The purpose of this research was to develop a simultaneous determination method for monoethanol-amine (MEA) and diethanolamine (DEA) in workplace air for risk assessment. The characteristics of the proposed method, such as recovery, quantitation limit, reproducibility and storage stability of the samples, were examined. An air sampling cassette containing two sulfuric acid-treated glass fiber filters was chosen as the sampler. The MEA and DEA were extracted from the sampler filters, derivatized with 9-fluorenylmethyloxycarbonyl chloride and then analyzed by a high-performance liquid Chromatograph equipped with a fluorescence detector or photo-diode array detector. The overall recoveries from spiked samplers were 86-99 and 88-99% for MEA and DEA, respectively. The recovery after 5 days of storage in a refrigerator exceeded 95%. The overall limits of quantitation were 0.750 and 0.100 jug/sample for MEA and DEA, respectively. The relative standard deviations, which represent the overall reproducibility defined as precision, were 0.3-1.6 and 0.4-5.7% for MEA and DEA, respectively. The proposed method enables 4-h personal exposure monitoring of MEA and DEA at concentrations equaling 1/3,000-2 times the threshold limit value-time-weighted average (TLV-TWA: 3 ppmfor MEA, 1 mg/m(3) for DEA) adopted by the American Conference of Governmental Industrial Hygienists and also by the Japan Society for Occupational Health. The method is useful for estimating worker exposure to MEA and DEA.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ethanolamine, puriss. p.a., ACS reagent, ≥99.0% (GC/NT)
Sigma-Aldrich
Diethanolamine, ACS reagent, ≥98.5%
Sigma-Aldrich
Ethanolamine, purified by redistillation, ≥99.5%
Sigma-Aldrich
Ethanolamine, ACS reagent, ≥99.0%
Sigma-Aldrich
Ethanolamine, ≥99%
Sigma-Aldrich
Ethanolamine, ≥98%
Sigma-Aldrich
Ethanolamine, liquid, BioReagent, suitable for cell culture, ≥98%
Sigma-Aldrich
Diethanolamine, reagent grade, ≥98.0%
Sigma-Aldrich
Diethanolamine, puriss. p.a., ACS reagent, ≥99.0% (GC)
Supelco
Ethanolamine, analytical standard
Supelco
Diethanolamine, analytical standard
Sigma-Aldrich
Diethanolamine, BioUltra, ≥99.5% (GC)
Supelco
Diethanolamine, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Diethanolamine hydrochloride, 98%