Methods for chromatographic removal of endotoxin.

Methods in molecular biology (Clifton, N.J.) (2012-06-28)
Adam J Lowe, Cameron L Bardliving, Carl A Batt
ABSTRACT

Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition as well as production scale. Here we present a robust method for endotoxin removal at the pilot production scale using fast protein liquid chromatography and buffers specifically engineered for endotoxin removal.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
IGEPAL® CA-630, for molecular biology
Sigma-Aldrich
IGEPAL® CA-630, viscous liquid
Sigma-Aldrich
Triton X-114, laboratory grade
Supelco
IGEPAL® CA-630
Sigma-Aldrich
Triton X-405 solution, 70% in H2O
Sigma-Aldrich
Triton X-114
Sigma-Aldrich
IGEPAL® CA-720, average Mn ~735
Sigma-Aldrich
Triton X-165 solution