• Housekeeping genes for phylogenetic analysis of eutherian relationships.

Housekeeping genes for phylogenetic analysis of eutherian relationships.

Molecular biology and evolution (2006-06-06)
Morgan Kullberg, Maria A Nilsson, Ulfur Arnason, Eric H Harley, Axel Janke

The molecular relationship of placental mammals has attracted great interest in recent years. However, 2 crucial and conflicting hypotheses remain, one with respect to the position of the root of the eutherian tree and the other the relationship between the orders Rodentia, Lagomorpha (rabbits, hares), and Primates. Although most mitochondrial (mt) analyses have suggested that rodents have a basal position in the eutherian tree, some nuclear data in combination with mt-rRNA genes have placed the root on the so-called African clade or on a branch that includes this clade and the Xenarthra (e.g., anteater and armadillo). In order to generate a new and independent set of molecular data for phylogenetic analysis, we have established cDNA sequences from different tissues of various mammalian species. With this in mind, we have identified and sequenced 8 housekeeping genes with moderately fast rate of evolution from 22 placental mammals, representing 11 orders. In order to determine the root of the eutherian tree, the same genes were also sequenced for 3 marsupial species, which were used as outgroup. Inconsistent with the analyses of nuclear + mt-rRNA gene data, the current data set did not favor a basal position of the African clade or Xenarthra in the eutherian tree. Similarly, by joining rodents and lagomorphs on the same basal branch (Glires hypothesis), the data set is also inconsistent with the tree commonly favored in mtDNA analyses. The analyses of the currently established sequences have helped examination of problematic parts in the eutherian tree at the same time as they caution against suggestions that have claimed that basal eutherian relationships have been conclusively settled.

Product Number
Product Description

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle, lyophilized powder, ≥75 units/mg protein
GAPDH, standard for protein electrophoresis
Malic Dehydrogenase from porcine heart, buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)
Malic Dehydrogenase from porcine heart, ≥600 units/mg protein (biuret), ammonium sulfate suspension
Glyceraldehyde-3-phosphate Dehydrogenase from human erythrocytes, lyophilized powder, 50-150 units/mg protein
Malic Dehydrogenase from bovine heart, ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)