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Removal of specific protein spots on the patterns of non-denaturing two-dimensional electrophoresis using protein A agarose and antibodies.

Journal of biochemical and biophysical methods (1998-11-24)
Y Shimazaki, S Ohara, T Manabe
ABSTRACT

Protein spots, such as immunoglobulin G (IgG), A (IgA) or alpha 2-macroglobulin, on the patterns of non-denaturing two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) were selectively removed by the application of protein A or antibody-conjugated protein A agarose to the first-dimension gel top at the cathode side. A protein residing at pI 5.8 and with a molecular mass of about 200 kDa was clearly detected when the IgG and IgA spots were removed from the 2-D PAGE pattern.

MATERIALS
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Product Description

Millipore
Protein A–Agarose Fast Flow, 50%, aqueous suspension