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Measuring Oxidative Stress in Caenorhabditis elegans: Paraquat and Juglone Sensitivity Assays.

Bio-protocol (2017-12-26)
Megan M Senchuk, Dylan J Dues, Jeremy M Van Raamsdonk
ABSTRACT

Oxidative stress has been proposed to be one of the main causes of aging and has been implicated in the pathogenesis of many diseases. Sensitivity to oxidative stress can be measured by quantifying survival following exposure to a reactive oxygen species (ROS)-generating compound such as paraquat or juglone. Sensitivity to oxidative stress is a balance between basal levels of ROS, the ability to detoxify ROS, and the ability to repair ROS-mediated damage.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Calcium chloride dihydrate, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Methyl viologen dichloride hydrate, 98%
Sigma-Aldrich
Potassium phosphate monobasic, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%