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  • FRAP: A Powerful Method to Evaluate Membrane Fluidity in Caenorhabditis elegans.

FRAP: A Powerful Method to Evaluate Membrane Fluidity in Caenorhabditis elegans.

Bio-protocol (2018-07-05)
Ranjan Devkota, Marc Pilon
ABSTRACT

FRAP (Fluorescence Recovery After Photobleaching) is probably the most direct method to investigate the dynamics of molecules in living cells. Here, we describe FRAP to quantify membrane fluidity in C. elegans. Using FRAP, we have shown that cold, glucose and exogenous saturated fatty acids can decrease the fluidity of cellular membranes in certain mutants.

MATERIALS
Product Number
Brand
Product Description

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α-D-Glucose, anhydrous, 96%
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