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  • Endogenous siRNAs promote proteostasis and longevity in germline-less Caenorhabditis elegans.

Endogenous siRNAs promote proteostasis and longevity in germline-less Caenorhabditis elegans.

eLife (2020-03-28)
Moran Cohen-Berkman, Reut Dudkevich, Shani Ben-Hamo, Alla Fishman, Yehuda Salzberg, Hiba Waldman Ben-Asher, Ayelet T Lamm, Sivan Henis-Korenblit
ABSTRACT

How lifespan and the rate of aging are set is a key problem in biology. Small RNAs are conserved molecules that impact diverse biological processes through the control of gene expression. However, in contrast to miRNAs, the role of endo-siRNAs in aging remains unexplored. Here, by combining deep sequencing and genomic and genetic approaches in Caenorhabditis elegans, we reveal an unprecedented role for endo-siRNA molecules in the maintenance of proteostasis and lifespan extension in germline-less animals. Furthermore, we identify an endo-siRNA-regulated tyrosine phosphatase, which limits the longevity of germline-less animals by restricting the activity of the heat shock transcription factor HSF-1. Altogether, our findings point to endo-siRNAs as a link between germline removal and the HSF-1 proteostasis and longevity-promoting somatic pathway. This establishes a role for endo siRNAs in the aging process and identifies downstream genes and physiological processes that are regulated by the endo siRNAs to affect longevity.

MATERIALS
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Product Description

Sigma-Aldrich
Monoclonal Anti-α-Tubulin antibody produced in mouse, clone B-5-1-2, ascites fluid
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Linoleic acid sodium salt, ≥98% (GC)