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Genomic Repeats Categorize Genes with Distinct Functions for Orchestrated Regulation.

Cell reports (2020-03-12)
J Yuyang Lu, Wen Shao, Lei Chang, Yafei Yin, Tong Li, Hui Zhang, Yantao Hong, Michelle Percharde, Lerui Guo, Zhongyang Wu, Lichao Liu, Wei Liu, Pixi Yan, Miguel Ramalho-Santos, Yujie Sun, Xiaohua Shen
ABSTRACT

Repetitive elements are abundantly distributed in mammalian genomes. Here, we reveal a striking association between repeat subtypes and gene function. SINE, L1, and low-complexity repeats demarcate distinct functional categories of genes and may dictate the time and level of gene expression by providing binding sites for different regulatory proteins. Importantly, imaging and sequencing analysis show that L1 repeats sequester a large set of genes with specialized functions in nucleolus- and lamina-associated inactive domains that are depleted of SINE repeats. In addition, L1 transcripts bind extensively to its DNA in embryonic stem cells (ESCs). Depletion of L1 RNA in ESCs leads to relocation of L1-enriched chromosomal segments from inactive domains to the nuclear interior and de-repression of L1-associated genes. These results demonstrate a role of L1 DNA and RNA in gene silencing and suggest a general theme of genomic repeats in orchestrating the function, regulation, and expression of their host genes.

MATERIALS
Product Number
Brand
Product Description

Supelco
N-(Propionyloxy)succinimide, for LC-MS derivatization, LiChropur, ≥95% (HPLC)
Sigma-Aldrich
Actinomycin D, from Streptomyces sp., ≥95% (HPLC)
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Kanamycin sulfate from Streptomyces kanamyceticus, Animal Component-free